Publication: Changes in circulating 25-hydroxyvitamin D according to vitamin D binding protein genotypes after vitamin D<inf>3</inf> or D<inf>2</inf> supplementation
Issued Date
2013-04-08
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14752891
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2-s2.0-84875688664
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Mahidol University
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SCOPUS
Bibliographic Citation
Nutrition Journal. Vol.12, No.1 (2013)
Suggested Citation
Hataikarn Nimitphong, Sunee Saetung, Suwannee Chanprasertyotin, La Or Chailurkit, Boonsong Ongphiphadhanakul Changes in circulating 25-hydroxyvitamin D according to vitamin D binding protein genotypes after vitamin D<inf>3</inf> or D<inf>2</inf> supplementation. Nutrition Journal. Vol.12, No.1 (2013). doi:10.1186/1475-2891-12-39 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/32395
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Title
Changes in circulating 25-hydroxyvitamin D according to vitamin D binding protein genotypes after vitamin D<inf>3</inf> or D<inf>2</inf> supplementation
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Abstract
Background: It is not known whether genetic variation in the vitamin D binding protein (DBP) influences 25-hydroxyvitamin D levels [25(OH)D] after vitamin D supplementation. We aimed to investigate the changes of total 25(OH)D, 25(OH)D3 and 25(OH)D2 in a Thai cohort, according to type of vitamin D supplement (vitamin D3 or D2) and DBP genotype, after receiving vitamin D3 or D2 for 3 months. Methods. Thirty-nine healthy subjects completed the study. All subjects received 400 IU of either vitamin D3 or D2, plus a calcium supplement, every day for 3 months. Total serum 25(OH)D, 25(OH)D3 and 25(OH)D2 were measured by LC-MS/MS. Individual genotyping of rs4588 in the DBP gene was performed using real-time PCR. Results: Vitamin D 3 supplementation of 400 IU/d increased 25(OH)D3 significantly (+16.2 ± 4.2 nmol/L, p <0.001). Vitamin D2 (400 IU/d) caused increased 25(OH)D2 levels (+22.0 ± 2.11 nmol/L, p <0.001), together with a decrease of 25(OH)D3 (-14.2 ± 2.0 nmol/L, p <0.001). At 3 month, subjects in vitamin D3 group tended to have higher total 25(OH)D levels than those in vitamin D 2 (67.8 ± 3.9 vs. 61.0 ± 3.0 nmol/L; p = 0.08). Subjects were then classified into two subgroups: homozygous for the DBP rs4588 C allele (CC), and the rest (CA or AA). With D3 supplementation, subjects with CA or AA alleles had significantly less increase in 25(OH)D 3 and total 25(OH)D when compared with those with the CC allele. However, no difference was found when the supplement was vitamin D2. Conclusion: Genetic variation in DBP (rs4588 SNP) influences responsiveness to vitamin D3 but not vitamin D2. © 2013 Nimitphong et al.; licensee BioMed Central Ltd.