Publication: A novel detection of a single plasmodium falciparum in infected blood
Issued Date
1991-02-28
Resource Type
ISSN
10902104
0006291X
0006291X
Other identifier(s)
2-s2.0-0026036365
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.175, No.1 (1991), 179-184
Suggested Citation
Witoon Tirasophon, Mathurose Ponglikitmongkol, Prapon Wilairat, Vichai Boonsaeng, Sakol Panyim A novel detection of a single plasmodium falciparum in infected blood. Biochemical and Biophysical Research Communications. Vol.175, No.1 (1991), 179-184. doi:10.1016/S0006-291X(05)81217-3 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21992
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
A novel detection of a single plasmodium falciparum in infected blood
Other Contributor(s)
Abstract
Detection of Plasmodium falciparum malaria by a specific DNA probe is a highly promising means for epidemiological surveillance of human malaria. However, none of presently available DNA probe methods could detect as little as a few parasites in infected blood. By amplification of a specific 206 base pairs P. falciparum DNA sequence using the polymerase chain reaction (PCR), as little as 0.01 picogram DNA or one-half of a parasite was sufficient for a specific detection. A PCR procedure for detection of P. falciparum in infected blood without prior DNA extraction was also developed which was sensitive for a single parasite. The procedure was simple and should be applicable for a large scale epidemiological study involving a very low parasitemia situation. © 1991 Academic Press, Inc.