Publication: Specific immune response and pathological findings in BALB/c mice inoculated with recombinat BCG expressing HIV-1 antigen
Issued Date
2005-03-01
Resource Type
ISSN
0125877X
Other identifier(s)
2-s2.0-20344390518
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.23, No.1 (2005), 41-51
Suggested Citation
Witthawat Wiriyarat, Sanya Sukpanichnant, Nopporn Sittisombut, Kruavon Balanchandra, Duanthanorm Promkhatkaew, Raywadee Butraporn, Ruengpung Sutthent, Jotika Boonlong, Kazuhiro Matsuo, Mitsuo Honda, Paijit Warachit, Pilaipan Puthavathana Specific immune response and pathological findings in BALB/c mice inoculated with recombinat BCG expressing HIV-1 antigen. Asian Pacific Journal of Allergy and Immunology. Vol.23, No.1 (2005), 41-51. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17061
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Title
Specific immune response and pathological findings in BALB/c mice inoculated with recombinat BCG expressing HIV-1 antigen
Abstract
Recombinant BCGs (rBCGs) containing extrachromosomal plasmids with different HIV-1 insert sequences: nef, env (V3J1 and E9Q), gag p17 or whole gag p55 were evaluated for their immunogenicity, safety and persistent infection in BALB/c mice. Animal injected with, rBCG-pIJKV3J1, rBCG-pSO gag p17 or rBCG-pSO gag p55 could elicit lymphocyte proliferation as tested by specific HIV-1 peptides or protein antigen. Inoculation with various concentration of rBCG-pSO gag p55 generated satisfactory specific lymphocyte proliferation in dose escalation trials. The rBCG-pSO gag p55 recovered from spleen tissues at different time interval post-inoculation could express the HIV protein as determined by ELISA p24 antigen detection kit. This result indicated that the extrachromosomal plasmid was stable and capable to express Gag protein. It was also demonstrated that rBCGs did not cause serious pathological change in the inoculated animals. The present study suggested the role of BCG as a potential vehicle for using in HIV vaccine development.