Publication:
Adiponectin mediated APPL1-AMPK signaling induces cell migration, MMP activation, and collagen remodeling in cardiac fibroblasts

dc.contributor.authorKeith Dadsonen_US
dc.contributor.authorHelen Chasiotisen_US
dc.contributor.authorSivaporn Wannaiampikulen_US
dc.contributor.authorRungsunn Tungtrongchitren_US
dc.contributor.authorAimin Xuen_US
dc.contributor.authorGary Sweeneyen_US
dc.contributor.otherYork Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherThe University of Hong Kongen_US
dc.date.accessioned2018-11-09T01:57:35Z
dc.date.available2018-11-09T01:57:35Z
dc.date.issued2014-01-01en_US
dc.description.abstractDefects in adiponectin action have been implicated in the development of cardiac dysfunction in obesity and diabetes. Cardiac fibroblasts play an important role in regulating extracellular matrix remodeling yet little is known regarding the direct effects of adiponectin on cardiac fibroblasts. In this study, we first demonstrated temporal relocalization of cellular APPL1 in response to adiponectin in primary cardiac fibroblasts and that siRNA-mediated knockdown of APPL1 attenuated stimulation of AMPK by adiponectin. The cell surface content of MT1-MMP and activation of MMP2 were induced by adiponectin and these responses were dependent on AMPK signaling. Enhanced MMP activity facilitated increased fibroblast migration in response to adiponectin which was also prevented by inhibition of AMPK, with no change in cell proliferation observed. Collagen and elastin immunofluorescence demonstrated reorganization of the extracellular matrix in accordance with increased MMP activity, whereas quantitative mRNA analysis, 3H-proline incorporation and picrosirius red assays showed no change in intracellular or extracellular total collagen levels in response to adiponectin. In summary, these data are the first to report the adiponectin stimulated APPL1-AMPK signaling axis in cardiac fibroblasts and characterize MT1-MMP translocation, MMP2 activity and cell migration as functional outcomes. These effects may be of significance in heart failure associated with obesity and diabetes. J. Cell. Biochem. 115: 785-793, 2014. © 2013 Wiley Periodicals, Inc. © 2013 Wiley Periodicals, Inc.en_US
dc.identifier.citationJournal of Cellular Biochemistry. Vol.115, No.4 (2014), 785-793en_US
dc.identifier.doi10.1002/jcb.24722en_US
dc.identifier.issn10974644en_US
dc.identifier.issn07302312en_US
dc.identifier.other2-s2.0-84892912997en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/33399
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84892912997&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleAdiponectin mediated APPL1-AMPK signaling induces cell migration, MMP activation, and collagen remodeling in cardiac fibroblastsen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84892912997&origin=inwarden_US

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