Publication: Akabane virus utilizes alternative endocytic pathways to entry into mammalian cell lines
Issued Date
2014-01-01
Resource Type
ISSN
13477439
09167250
09167250
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2-s2.0-84913582072
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Veterinary Medical Science. Vol.76, No.11 (2014), 1471-1478
Suggested Citation
Norasuthi Bangphoomi, Akiko Takenaka-Uema, Tatsuki Sugi, Kentaro Kat O, Hiroomi Akashi, Taisuke Horimoto Akabane virus utilizes alternative endocytic pathways to entry into mammalian cell lines. Journal of Veterinary Medical Science. Vol.76, No.11 (2014), 1471-1478. doi:10.1292/jvms.14-0155 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/34964
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Title
Akabane virus utilizes alternative endocytic pathways to entry into mammalian cell lines
Abstract
©2014 The Japanese Society of Veterinary Science. The entry mechanisms of Akabane virus (AKAV), Bunyaviridae family, have not yet been determined. In this study, chemical inhibitors were used to analyze endocytic mechanisms during AKAV infection of mammalian cell lines. The analyses using drug treatments followed by quantitative measurement of viral RNA and N protein revealed that AKAV enters non-bovine-derived cell lines (Vero, HmLu-1 and BHK cells) in a manner indicative of clathrin endocytosis. By contrast, AKAV infection in bovine-derived cell lines (LB9.K and MDBK cells) is independent of this pathway. Further analyses indicated that AKAV entry into bovine cell lines involves a non-clathrin, non-caveolae endocytic pathway that is dependent on dynamin. We conclude that although both cell types require a low pH for AKAV penetration, AKAV utilizes alternative entry pathways into mammalian cell lines.