Publication:
Combined effects of 17β-estradiol and exercise training on cardiac apoptosis in ovariectomized rats

dc.contributor.authorYi Yuan Linen_US
dc.contributor.authorJwo Sheng Chenen_US
dc.contributor.authorXu Bo Wuen_US
dc.contributor.authorWoei Cherng Shyuen_US
dc.contributor.authorRungchai Chaunchaiyakulen_US
dc.contributor.authorXian Li Zhaoen_US
dc.contributor.authorChia Hua Kuoen_US
dc.contributor.authorYu Jung Chengen_US
dc.contributor.authorAi Lun Yangen_US
dc.contributor.authorShin Da Leeen_US
dc.contributor.otherUniversity of Taipeien_US
dc.contributor.otherAsia University Taiwanen_US
dc.contributor.otherChina Medical University Hospital Taichungen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherShanghai University of Traditional Chinese Medicineen_US
dc.contributor.otherChina Medical University Taichungen_US
dc.date.accessioned2019-08-23T10:13:08Z
dc.date.available2019-08-23T10:13:08Z
dc.date.issued2018-12-01en_US
dc.description.abstract© 2018 Lin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Background The purpose of this study was to investigate the combined 17β-estradiol (E2) and exercise training on cardiac pro-survival and anti-apoptotic pathways in ovariectomized rats. Methods Fifty-six female Sprague–Dawley rats were divided into a sham-operated (Sham), a bilaterally ovariectomized (OVX), an OVX treated with E2 (OVX-E2; 10μg/kg/day), and an OVX with E2 and treadmill exercise training (OVX-E2-EX; 60 min/day, 5 days/week) for 10 weeks. Following 10 weeks of exercise training, rat hearts were isolated for the evaluation of Histopathological analysis, TUNEL assay, and Western blotting. Results The protein levels of estrogen receptor α (ERα), estrogen receptor β (ERβ), insulin-like growth factor 1 (IGF-1), IGF-1 receptor (IGF-1R), phospho-phosphatidylinositol 3-kinase (p-PI3K) (estrogen receptors/IGF-1-related survival pathway) were significantly increased in either the OVX-E2 or OVX-E2-EX group when compared with the OVX group. The protein levels of B-cell lymphoma 2 (Bcl-2), B-cell lymphoma-extra-large (Bcl-xL) and phosphory-lated-Bad (p-Bad) (Bcl-2 family survival pathway) were significantly increased in the OVX-E2-EX group when compared with the OVX group. Only the p-Bad was significantly increased in the OVX-E2 group when compared with the OVX group. The protein levels of truncation of Bid (t-Bid), Bcl-2-associated death promotor (Bad), Bcl-2-associated X protein (Bax), Cytochrome c, caspases-9, and caspases-3 (mitochondria-dependent apoptotic pathway), as well as the protein levels of tumor necrosis factor-α (TNF-α), Fas ligand, Fas receptors, Fas-associated death domain (FADD), activated caspase-8 and activated caspase-3 (Fas receptor–dependent apoptotic pathway) were significantly decreased in either the OVX-E2 or OVX-E2-EX group when compared with the OVX group. Furthermore, when compared with the OVX-E2 group, the protein levels of ERβ, IGF-1, IGF-1R, Bcl-2 and Bcl-xL were further enhanced in the OVX-E2-EX group as well as the protein levels of Cytochrome c, Fas receptors, FADD, activated caspase-8, activated caspase-9 and activated caspase-3 were further decreased in the OVX-EX-E2 group. Conclusions Combined E2 and exercise training exhibited a positive effect of protection on ovariectomy-induced cardiac apoptosis by enhancing ERβ-related survival pathways, which might provide a more effective therapeutic effect on cardiac protection in bilaterally oophorectomized or menopausal women than E2 treatment only.en_US
dc.identifier.citationPLoS ONE. Vol.13, No.12 (2018)en_US
dc.identifier.doi10.1371/journal.pone.0208633en_US
dc.identifier.issn19326203en_US
dc.identifier.other2-s2.0-85058923945en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/44639
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85058923945&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleCombined effects of 17β-estradiol and exercise training on cardiac apoptosis in ovariectomized ratsen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85058923945&origin=inwarden_US

Files

Collections