Publication: Peptide-based delivery of oligonucleotides across blood-brain barrier model
Issued Date
2014-01-01
Resource Type
ISSN
15733904
15733149
15733149
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2-s2.0-84899483274
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Peptide Research and Therapeutics. Vol.20, No.2 (2014), 169-178
Suggested Citation
Artita Srimanee, Jakob Regberg, Mattias Hallbrink, Kaido Kurrikoff, Kadi Liis Veiman, Opa Vajragupta, Ülo Langel Peptide-based delivery of oligonucleotides across blood-brain barrier model. International Journal of Peptide Research and Therapeutics. Vol.20, No.2 (2014), 169-178. doi:10.1007/s10989-013-9378-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33458
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Title
Peptide-based delivery of oligonucleotides across blood-brain barrier model
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Abstract
Delivery of pharmaceutical agents across a blood-brain barrier (BBB) is a challenge for brain cancer therapy. In this study, an in vitro BBB model was utilized to study the delivery of oligonucleotides across brain endothelial cells targeting to glioma cells in a Transwell™ setup. A series of novel peptides were synthesized by covalent conjugation of cell-penetrating peptides with targeting peptides for delivery of gene-based therapeutics. These peptides were screened for passage across the Transwell™ and we found the most efficient peptide PepFect32 from originating PepFect 14 coupled with the targeting peptide angiopep-2. PepFect32/pDNA nanocomplexes exhibited high transcytosis across the BBB in vitro model and the highest transfection efficiency to glioma cells. In conclusion, PepFect32 revealed the most efficient peptide-based vector for pDNA delivery across in vitro BBB model. © 2013 Springer Science+Business Media.