Publication: Cytotoxicity of three light-cured orthodontic adhesives
Issued Date
2018-01-01
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ISSN
10139826
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2-s2.0-85054878348
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Mahidol University
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SCOPUS
Bibliographic Citation
Key Engineering Materials. Vol.777 KEM, (2018), 582-586
Suggested Citation
Natthasit Pudpong, Niwat Anuwongnukroh, Surachai Dechkunakorn, Wassana Wichai, Peerapong Tua-ngam Cytotoxicity of three light-cured orthodontic adhesives. Key Engineering Materials. Vol.777 KEM, (2018), 582-586. doi:10.4028/www.scientific.net/KEM.777.582 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45832
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Title
Cytotoxicity of three light-cured orthodontic adhesives
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Abstract
© 2018 Trans Tech Publications, Switzerland. Objective: The aim of this study was to evaluate the cytotoxic effects of three commercial light-cured orthodontic adhesives. Materials and methods: The potential cytotoxic effects of three types of orthodontic adhesives, Grengloo, Green Glue, and Transbond XT, were tested on L929 cell culture. The cell line was grown in 96-well tissue culture plates (1×105 cells/mm3). Thin resin discs weighing 0.4, 0.6, 0.8, 0.8, and 0.8 gram of each material were prepared and aged for 1, 3, 6, 8, and 10 days, respectively, in Minimum Essential Medium (MEM) at 37°C with 5% CO2 at 100% humidity. Cell viability was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay according to ISO 10993-5: 2009 (E). The differences among the groups was statically analyzed by independent paired t-test (α = 0.05). Results: After 1 day of storage, all adhesive systems showed cytotoxic effects. However, ageing tended to considerably reduce the cytotoxicity of Green Glue. Grengloo was essentially non-cytotoxic day 3 onwards, while Green Glue and Transbond XT exhibited potential cytotoxicity at all times of the experiment. Conclusion: All tested light-cured orthodontic adhesives had cytotoxic potential during the first day. Grengloo had the highest cell viability, whereas, Green Glue had the lowest.