Publication: Blood-induced differential gene expression in Anopheles dirus evaluated using RNA sequencing
Issued Date
2018-12-01
Resource Type
ISSN
13652915
0269283X
0269283X
Other identifier(s)
2-s2.0-85055977950
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Medical and Veterinary Entomology. Vol.32, No.4 (2018), 399-406
Suggested Citation
W. Mongkol, W. Nguitragool, J. Sattabongkot, A. Kubera Blood-induced differential gene expression in Anopheles dirus evaluated using RNA sequencing. Medical and Veterinary Entomology. Vol.32, No.4 (2018), 399-406. doi:10.1111/mve.12310 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/44623
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Blood-induced differential gene expression in Anopheles dirus evaluated using RNA sequencing
Author(s)
Other Contributor(s)
Abstract
© 2018 The Royal Entomological Society Malaria parasites are transmitted through blood feeding by female Anopheline mosquitoes. Unveiling the blood-feeding process will improve understanding of vector biology. Anopheles dirus (Diptera: Culicidae) is one of the primary malaria vectors in the Greater Mekong Subregion, the epicentre of malaria drug resistance. In this study, differential gene expression between sugar- and blood-fed An. dirus was investigated by RNA sequencing (RNA-seq). A total of 589 transcripts were found to be upregulated and 703 transcripts downregulated as a result of blood feeding. Transcriptional differences were found in genes involved in blood digestion, peritrophic matrix formation, oogenesis and vitellogenesis. The expression levels of several genes were validated by quantitative reverse transcription polymerase chain reaction. The present results provide better understanding of An. dirus biology in relation to its blood feeding.