Publication: High-performance liquid chromatography of aflatoxins in human urine
Issued Date
1983-01-01
Resource Type
ISSN
03784347
Other identifier(s)
2-s2.0-0020619999
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Chromatography B: Biomedical Sciences and Applications. Vol.275, No.C (1983), 387-393
Suggested Citation
Tongtavuch Anukarahanonta, Chutima Chudhabuddhi High-performance liquid chromatography of aflatoxins in human urine. Journal of Chromatography B: Biomedical Sciences and Applications. Vol.275, No.C (1983), 387-393. doi:10.1016/S0378-4347(00)84384-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/30464
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Title
High-performance liquid chromatography of aflatoxins in human urine
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Abstract
A method was developed for the extraction and determination of unconjugated aflatoxins in human urine by high-performance liquid chromatography. The analysis is based on the elimination of lipid-soluble constituents other than unconjugated aflatoxins in urine by light petroleum extraction. The unconjugated aflatoxins were subsequently extracted from the aqueous phase with chloroform-acetone. Chromatography was performed isocratically with a silica column at 40°C. The resolved aflatoxins were detected and identified by ultraviolet and fluorometric detectors. The recoveries of aflatoxins B1and G1added prior to the extraction were 72% and 83%, respectively. This procedure is simple, sensitive and practically useful for epidemiological survey of unconjugated aflatoxins in human urine from areas with a high risk of aflatoxin consumption. © 1983.