Publication: Detection of Alveolar Rhabdomyosarcoma in Pleural Fluid with Immunocytochemistry on Cell Block and Determination of PAX/FKHR fusion mRNA by Reverse Transcription-Polymerase Chain Reaction
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Issued Date
2011-11-01
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ISSN
01252208
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2-s2.0-84856984965
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol.94, No.11 (2011), 1394-1398
Suggested Citation
Ruchchadol Sawangpanich, Noppadol Larbcharoensub, Artit Jinawath, Atcharaporn Pongtippan, Usanarat Anurathapan, Suradej Hongeng Detection of Alveolar Rhabdomyosarcoma in Pleural Fluid with Immunocytochemistry on Cell Block and Determination of PAX/FKHR fusion mRNA by Reverse Transcription-Polymerase Chain Reaction. Journal of the Medical Association of Thailand. Vol.94, No.11 (2011), 1394-1398. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/12236
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Title
Detection of Alveolar Rhabdomyosarcoma in Pleural Fluid with Immunocytochemistry on Cell Block and Determination of PAX/FKHR fusion mRNA by Reverse Transcription-Polymerase Chain Reaction
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Abstract
Background: Alveolar rhabdomyosarcoma is a primitive malignant round cell neoplasm, which shows skeletal muscle differentiation. Although their histopathologic and immunohistochemical findings are well known, the cytology, immunocytochemistry and molecular study on pleural effusion have not been well documented. Objective: To apply molecular method in the diagnosis and monitoring of alveolar rhabdomyosarcoma. Case Report: The case of a 14-year-old Thai male, who presented with dyspnea and left pleural effusion. Computed tomography of the chest and abdomen showed a huge heterogeneous enhancing mass at the left retroperitoneum. Pleural fluid cytology showed malignant small round blue cells. Immunocytochemical stains on cell block material showed positive reactivity to vimentin, sarcomeric actin, desmin, MyoD1, myogenin, and CD56 in round cell tumor. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated PAX/FKHR fusion transcript. The patient received chemotherapeutic regimen for advanced-stage rhabdomyosarcoma. Finally, he succumbed to the disease, thirteen months after the diagnosis. Conclusion: Immunocytochemistry on cell block in conjunction with determination of PAX/FKHR fusion mRNA by RT-PCR is a molecular method in the diagnosis and monitoring of alveolar rhabdomyosarcoma in pleural fluid.