Publication: Suitability of capillary blood for quantitative assessment of G6PD activity and performances of G6PD point-of-care tests
Issued Date
2015-01-01
Resource Type
ISSN
00029637
Other identifier(s)
2-s2.0-84926323959
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.92, No.4 (2015), 818-824
Suggested Citation
Germana Bancone, Cindy S. Chu, Nongnud Chowwiwat, Raweewan Somsakchaicharoen, Pornpimon Wilaisrisak, Prakaykaew Charunwatthana, Pooja Bansil, Sarah McGray, Gonzalo J. Domingo, François H. Nosten Suitability of capillary blood for quantitative assessment of G6PD activity and performances of G6PD point-of-care tests. American Journal of Tropical Medicine and Hygiene. Vol.92, No.4 (2015), 818-824. doi:10.4269/ajtmh.14-0696 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/36164
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Suitability of capillary blood for quantitative assessment of G6PD activity and performances of G6PD point-of-care tests
Other Contributor(s)
Abstract
Copyright © 2015 by The American Society of Tropical Medicine and Hygiene. The use of primaquine and other 8-aminoquinolines for malaria elimination is hampered by, among other factors, the limited availability of point-of-care tests for the diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency. Historically, the most used source of blood for G6PD analyses is venous blood, whereas diagnostic devices used in the field require the use of capillary blood; data have shown that the two sources of blood often differ with respect to hemoglobin concentration and number of red blood cells. Therefore, we have analyzed, in both capillary and venous blood drawn from the same healthy donors, the correlation of G6PD activity assessed by two qualitative tests (the Fluorescent Spot test and the CareStart test) with the gold standard quantitative spectrophotometric assay. Results obtained on 150 subjects with normal, intermediate, and deficient G6PD phenotypes show that, although differences exist between the aforementioned characteristics in capillary and venous blood, these do not impact on the quantitative assessment of G6PD activity after corrected for hemoglobin concentration or red blood cell count. Furthermore, we have assessed the sensitivity and specificity of the two qualitative tests against the gold standard spectrophotometric assay at different activity thresholds of residual enzymatic activity in both blood sources.
