Publication: Antiproliferation, antioxidation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line
Issued Date
2004-01-01
Resource Type
ISSN
03788741
Other identifier(s)
2-s2.0-0345862032
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Ethnopharmacology. Vol.90, No.1 (2004), 161-166
Suggested Citation
Primchanien Moongkarndi, Nuttavut Kosem, Sineenart Kaslungka, Omboon Luanratana, Narongchai Pongpan, Neelobol Neungton Antiproliferation, antioxidation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line. Journal of Ethnopharmacology. Vol.90, No.1 (2004), 161-166. doi:10.1016/j.jep.2003.09.048 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21815
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Title
Antiproliferation, antioxidation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line
Abstract
This study was designed to determine the antiproliferative, apoptotic and antioxidative properties of crude methanolic extract (CME) from the pericarp of Garcinia mangostana (family Guttiferae) using human breast cancer (SKBR3) cell line as a model system. SKBR3 cells were cultured in the presence of CME at various concentrations (0-50μg/ml) for 48h and the percentage of cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-di phenyl tetrazolium bromide (MTT) assay. CME showed a dose-dependent inhibition of cell proliferation with ED50of 9.25±0.64μg/ml. We found that antiproliferative effect of CME was associated with apoptosis on breast cancer cell line by determinations of morphological changes and oligonucleosomal DNA fragments. In addition, CME at various concentrations and incubation times were also found to inhibit ROS production. These investigations suggested that the methanolic extract from the pericarp of Garcinia mangostana had strong antiproliferation, potent antioxidation and induction of apoptosis. Thus, it indicates that this substance can show different activities and has potential for cancer chemoprevention which were dose dependent as well as exposure time dependent. © 2003 Elsevier Ireland Ltd. All rights reserved.