Publication: Development of Pyrosequencing Method for Detection of UGT1A1 Polymorphisms in Thai Colorectal Cancers
Issued Date
2016-01-01
Resource Type
ISSN
10982825
08878013
08878013
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2-s2.0-84954076213
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Clinical Laboratory Analysis. Vol.30, No.1 (2016), 84-89
Suggested Citation
Chonlaphat Sukasem, Chalirmporn Atasilp, Pichai Chansriwong, Montri Chamnanphon, Apichaya Puangpetch, Ekapob Sirachainan Development of Pyrosequencing Method for Detection of UGT1A1 Polymorphisms in Thai Colorectal Cancers. Journal of Clinical Laboratory Analysis. Vol.30, No.1 (2016), 84-89. doi:10.1002/jcla.21820 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/43133
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Title
Development of Pyrosequencing Method for Detection of UGT1A1 Polymorphisms in Thai Colorectal Cancers
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Abstract
© 2016 Wiley Periodicals, Inc. Background: UGT1A1 is a polymorphic enzyme that has been associated with irinotecan drug metabolisms. We developed a pyrosequencing method to detect allele frequency and genotype of UGT1A1 polymorphisms (UGT1A1*28 and UGT1A1*6) in Thai colorectal cancer patients. Method: A pyrosequencing method was designed to determine UGT1A1 genetic polymorphisms including UGT1A1*28 (A[TA]7TAA) and UGT1A1*6 (211G>A) in 91 Thai colorectal cancers. Result: Genotyping by the pyrosequencing technique was 100% concordant with capillary electrophoresis sequencing. The allele frequencies for UGT1A1 genetic polymorphisms were *1/*1 (54.95%), *1/*6 (13.19%), *1/*28 (25.27%), *28/*6 (4.40%), and *28/*28 (2.20%). No homozygous mutation UGT1A1*6 was found in our population. Conclusions: We developed a rapid, reliable, more cost-effective, and simple assay to detect UGT1A1 genetic polymorphisms in routine practice before initiating irinotecan therapy. The UGT1A1*28 and UGT1A1*6 alleles were found to be similar in the Asian populations.