Publication: Investigation of FoxO3 dynamics during erythroblast development in β-thalassemia major
Issued Date
2017-11-01
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ISSN
19326203
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2-s2.0-85032802060
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Mahidol University
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SCOPUS
Bibliographic Citation
PLoS ONE. Vol.12, No.11 (2017)
Suggested Citation
Naruchit Thanuthanakhun, Lalana Nuntakarn, Somponnat Sampattavanich, Usanarat Anurathapan, Suphanun Phuphanitcharoenkun, Savichaya Pornpaiboonstid, Suparerk Borwornpinyo, Suradej Hongeng Investigation of FoxO3 dynamics during erythroblast development in β-thalassemia major. PLoS ONE. Vol.12, No.11 (2017). doi:10.1371/journal.pone.0187610 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41308
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Title
Investigation of FoxO3 dynamics during erythroblast development in β-thalassemia major
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Abstract
© 2017 Thanuthanakhun et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The FoxO3 transcription factor is a key regulator of oxidative stress and erythroid maturation during erythropoiesis. In this study, we explored the involvement of FoxO3 in severe β-thalassemia. Using primary CD34+hematopoietic progenitor cells from patients with β-thalassemia major, we successfully developed an in vitro model of ineffective erythropoiesis. Based on this model, FoxO3 activity was quantified in single cells using high throughput imaging flow cytometry. This study revealed a significant reduction of FoxO3 activity during the late stage of erythroblast differentiation in β-thalassemia, in contrast to erythropoiesis in normal cells that maintain persistent activation of FoxO3. In agreement with the decreased FoxO3 activity in β-thalassemia, the expression of FoxO3 target genes was also found to decrease, concurrent with elevated phosphorylation of AKT, most clearly at the late stage of erythroid differentiation. Our findings provide further evidence for the involvement of FoxO3 during terminal erythropoiesis and confirm the modulation of the PI3K/AKT pathway as a potential therapeutic strategy for β-thalassemia.