Publication: Sequential injection analysis with electrochemical detection as a tool for economic and rapid evaluation of total antioxidant capacity
Issued Date
2011-06-15
Resource Type
ISSN
00399140
Other identifier(s)
2-s2.0-79958147232
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Talanta. Vol.84, No.5 (2011), 1350-1354
Suggested Citation
S. Chan-Eam, S. Teerasong, K. Damwan, D. Nacapricha, R. Chaisuksant Sequential injection analysis with electrochemical detection as a tool for economic and rapid evaluation of total antioxidant capacity. Talanta. Vol.84, No.5 (2011), 1350-1354. doi:10.1016/j.talanta.2011.02.043 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11711
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Sequential injection analysis with electrochemical detection as a tool for economic and rapid evaluation of total antioxidant capacity
Abstract
This work presents a new flow-based coupled electrochemical technique for evaluation of "total antioxidant capacity (TAC)". A sequential injection (SI) with amperometric detection was applied to the TAC analysis of commercial instant ginger infusion beverages using 2,2′-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. Besides having chromogenic properties, the ABTS reagent behaves as an electroactive species at the glassy carbon electrode in phosphate buffer pH 7.0, the decrease of the cathodic current signal of the ABTS + radical after reaction with antioxidants can be monitored. The SI system, furnished with an in-house electrochemical detection cell (ECD), was optimized with respect to the applied potential, sample and reagent volume, and fl ow rate to the detector. Gallic acid was used as the standard antioxidant and the capacity was reported as gallic acid equivalent (GAE) unit. TAC measurements of ginger infusions at the optimum condition were performed using the proposed technique and also with the classical batch spectrophotometric ABTS assay. TAC values obtained from our method and the standard method are in good agreement (r 2 = 0.956). The SI-amperometric technique provided satisfactory precision (4.11% RSD) with rapid sample throughput (40 samples h -1 ). Also using this method, the consumption of the expensive ABTS reagent was greatly reduced. © 2011 Elsevier B.V. All rights reserved.