Publication: Engineering of a novel chimera of superoxide dismutase and Vitreoscilla Hemoglobin for rapid detoxification of reactive oxygen species
Issued Date
2010-12-01
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ISSN
13891723
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2-s2.0-78449264854
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Bioscience and Bioengineering. Vol.110, No.6 (2010), 633-637
Suggested Citation
Chartchalerm Isarankura-Na-Ayudhya, Sakda Yainoy, Tanawut Tantimongcolwat, Leif Bülow, Virapong Prachayasittikul Engineering of a novel chimera of superoxide dismutase and Vitreoscilla Hemoglobin for rapid detoxification of reactive oxygen species. Journal of Bioscience and Bioengineering. Vol.110, No.6 (2010), 633-637. doi:10.1016/j.jbiosc.2010.07.001 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28580
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Title
Engineering of a novel chimera of superoxide dismutase and Vitreoscilla Hemoglobin for rapid detoxification of reactive oxygen species
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Abstract
The genes encoding human manganese superoxide dismutase (MnSOD) and Vitreoscilla hemoglobin (VHb) were fused in-frame to generate a bifunctional enzyme that possessed MnSOD and peroxidase-like activities. At neutral pH, the coupling of the SOD and peroxidase reactions revealed that the bifunctional enzyme exhibited a 2.5 times shorter transient period and a 1.67 times higher reaction rate at steady-state conditions. Furthermore, the catalytic rate of the bifunctional enzyme was not affected as much by the external H2O2scavenger catalase. This indicates that the bifunctional protein possesses a greater antioxidant capability, which is possibly due to the close proximity between the active site of MnSOD and the heme moiety of VHb. Our findings not only provide insight into the synergistic functions of SOD and peroxidase but also could potentially be used to develop novel therapeutic agents with more efficient O2carrying capability. © 2010 The Society for Biotechnology, Japan.