Publication: Human single-chain antibodies that neutralize homologous and heterologous strains and clades of influenza A virus subtype H5N1
Issued Date
2009-06-29
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ISSN
13596535
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2-s2.0-67149113906
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Mahidol University
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SCOPUS
Bibliographic Citation
Antiviral Therapy. Vol.14, No.2 (2009), 221-230
Suggested Citation
Santi Maneewatch, Jeeraphong Thanongsaksrikul, Thaweesak Songserm, Kanyarat Thueng-In, Kasem Kulkeaw, Umaporn Thathaisong, Potjanee Srimanote, Pongsri Tongtawe, Pramuan Tapchaisri, Wanpen Chaicumpa Human single-chain antibodies that neutralize homologous and heterologous strains and clades of influenza A virus subtype H5N1. Antiviral Therapy. Vol.14, No.2 (2009), 221-230. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28041
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Title
Human single-chain antibodies that neutralize homologous and heterologous strains and clades of influenza A virus subtype H5N1
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Abstract
Background: Human antibodies that interfere with the biological activity of haemagglutinins (HAs) of influenza viruses have high potential as an antiviral agent. Methods: Human single-chain antibody fragments (HuScFv) to recombinant and native HAs of the influenza virus H5N1 subtype were produced using a human antibody phage display library with the intention to increase the therapeutic arsenal against this highly pathogenic virus. Results: The HuScFv inhibited HA activity and neutralized infectivity of both homologous and heterologous strains and clades of the H5N1 subtype in Madin-Darby canine kidney cell cultures. Intraperitoneally injected HuScFv also mediated immunotherapeutic protection in mice that were intranasally challenged with highly pathogenic H5N1 viruses belonging to different strains and clades. Conclusions: Our data indicate that it might be worth pursuing these HuScFv further for future consideration as candidates for influenza intervention and treatment. © 2009 International Medical Press.