Publication: Native-like SARS-CoV-2 Spike Glycoprotein Expressed by ChAdOx1 nCoV-19/AZD1222 Vaccine
Issued Date
2021-04-28
Resource Type
ISSN
23747951
23747943
23747943
Other identifier(s)
2-s2.0-85105109368
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
ACS Central Science. Vol.7, No.4 (2021), 594-602
Suggested Citation
Yasunori Watanabe, Luiza Mendonça, Elizabeth R. Allen, Andrew Howe, Mercede Lee, Joel D. Allen, Himanshi Chawla, David Pulido, Francesca Donnellan, Hannah Davies, Marta Ulaszewska, Sandra Belij-Rammerstorfer, Susan Morris, Anna Sophia Krebs, Wanwisa Dejnirattisai, Juthathip Mongkolsapaya, Piyada Supasa, Gavin R. Screaton, Catherine M. Green, Teresa Lambe, Peijun Zhang, Sarah C. Gilbert, Max Crispin Native-like SARS-CoV-2 Spike Glycoprotein Expressed by ChAdOx1 nCoV-19/AZD1222 Vaccine. ACS Central Science. Vol.7, No.4 (2021), 594-602. doi:10.1021/acscentsci.1c00080 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/76537
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Native-like SARS-CoV-2 Spike Glycoprotein Expressed by ChAdOx1 nCoV-19/AZD1222 Vaccine
Author(s)
Yasunori Watanabe
Luiza Mendonça
Elizabeth R. Allen
Andrew Howe
Mercede Lee
Joel D. Allen
Himanshi Chawla
David Pulido
Francesca Donnellan
Hannah Davies
Marta Ulaszewska
Sandra Belij-Rammerstorfer
Susan Morris
Anna Sophia Krebs
Wanwisa Dejnirattisai
Juthathip Mongkolsapaya
Piyada Supasa
Gavin R. Screaton
Catherine M. Green
Teresa Lambe
Peijun Zhang
Sarah C. Gilbert
Max Crispin
Luiza Mendonça
Elizabeth R. Allen
Andrew Howe
Mercede Lee
Joel D. Allen
Himanshi Chawla
David Pulido
Francesca Donnellan
Hannah Davies
Marta Ulaszewska
Sandra Belij-Rammerstorfer
Susan Morris
Anna Sophia Krebs
Wanwisa Dejnirattisai
Juthathip Mongkolsapaya
Piyada Supasa
Gavin R. Screaton
Catherine M. Green
Teresa Lambe
Peijun Zhang
Sarah C. Gilbert
Max Crispin
Abstract
Vaccine development against the SARS-CoV-2 virus focuses on the principal target of the neutralizing immune response, the spike (S) glycoprotein. Adenovirus-vectored vaccines offer an effective platform for the delivery of viral antigen, but it is important for the generation of neutralizing antibodies that they produce appropriately processed and assembled viral antigen that mimics that observed on the SARS-CoV-2 virus. Here, we describe the structure, conformation, and glycosylation of the S protein derived from the adenovirus-vectored ChAdOx1 nCoV-19/AZD1222 vaccine. We demonstrate native-like post-translational processing and assembly, and reveal the expression of S proteins on the surface of cells adopting the trimeric prefusion conformation. The data presented here confirm the use of ChAdOx1 adenovirus vectors as a leading platform technology for SARS-CoV-2 vaccines.