Publication: Alteration of egg activation by protease inhibitors in the black tiger shrimp, Penaeus monodon
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Issued Date
2015-09-25
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07924259
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2-s2.0-84945242353
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Mahidol University
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SCOPUS
Bibliographic Citation
Invertebrate Reproduction and Development. Vol.59, No.4 (2015), 206-211
Suggested Citation
Piyada Ngernsoungnern, Prapee Sretarugsa, Apichart Ngernsoungnern Alteration of egg activation by protease inhibitors in the black tiger shrimp, Penaeus monodon. Invertebrate Reproduction and Development. Vol.59, No.4 (2015), 206-211. doi:10.1080/07924259.2015.1086827 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/35095
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Title
Alteration of egg activation by protease inhibitors in the black tiger shrimp, Penaeus monodon
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Abstract
© 2015 Taylor & Francis. In penaeoid shrimp, contact of spawned eggs with seawater induces egg activation. However, little is known about the factors that influence egg activation in Penaeus monodon. Therefore, the main objective of the present study was to determine whether shrimp-produced proteases that are released in seawater are essential for egg activation. Female shrimp were allowed to spawn in artificial seawater containing protease inhibitors. It was shown that 4-amidinophenylmethanesulfonyl fluoride hydrochloride (APMSF) and soybean trypsin inhibitor (SBTI) inhibited egg activation. High doses of APMSF and SBTI induced only 1–2% complete egg activation. Moreover, when the APMSF- and SBTI- treated eggs were subsequently washed, egg activation did not resume. In contrast, other protease inhibitors, pepstatin A, E-64, and ethylene glycol tetraacetic acid, did not inhibit egg activation, as evident by approximately 98% complete activation. Our results suggest that serine proteases, which are most likely trypsin-like proteases, released in seawater may be involved in egg activation of P. monodon.