Publication:
A Single Multilocus Sequence Typing (MLST) Scheme for Seven Pathogenic Leptospira Species

Issued Date

2013-01-01

Resource Type

Article

ISSN

19352735
19352727

DOI

10.1371/journal.pntd.0001954

Other identifier(s)

2-s2.0-84873493253

Rights

Mahidol University

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SCOPUS

Bibliographic Citation

PLoS Neglected Tropical Diseases. Vol.7, No.1 (2013)

Suggested Citation

Siriphan Boonsilp, Janjira Thaipadungpanit, Premjit Amornchai, Vanaporn Wuthiekanun, Mark S. Bailey, Matthew T G Holden, Cuicai Zhang, Xiugao Jiang, Nobuo Koizumi, Kyle Taylor, Renee Galloway, Alex R. Hoffmaster, Scott Craig, Lee D. Smythe, Rudy A. Hartskeerl, Nicholas P. Day, Narisara Chantratita, Edward J. Feil, David M. Aanensen, Brian G. Spratt, Sharon J. Peacock A Single Multilocus Sequence Typing (MLST) Scheme for Seven Pathogenic Leptospira Species. PLoS Neglected Tropical Diseases. Vol.7, No.1 (2013). doi:10.1371/journal.pntd.0001954 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/32708

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Title

A Single Multilocus Sequence Typing (MLST) Scheme for Seven Pathogenic Leptospira Species

Author(s)

Siriphan Boonsilp
 Janjira Thaipadungpanit
 Premjit Amornchai
 Vanaporn Wuthiekanun
 Mark S. Bailey
 Matthew T G Holden
 Cuicai Zhang
 Xiugao Jiang
 Nobuo Koizumi
 Kyle Taylor
 Renee Galloway
 Alex R. Hoffmaster
 Scott Craig
 Lee D. Smythe
 Rudy A. Hartskeerl
 Nicholas P. Day
 Narisara Chantratita
 Edward J. Feil
 David M. Aanensen
 Brian G. Spratt
 Sharon J. Peacock

Other Contributor(s)

Mahidol University
 Heartlands Hospital
 Wellcome Trust Sanger Institute
 Chinese Center for Disease Control and Prevention
 National Institute of Infectious Diseases
 Hokkaido University
 National Center for Emerging and Zoonotic Infectious Diseases
 Queensland Health
 Royal Tropical Institute - KIT
 Nuffield Department of Clinical Medicine
 University of Bath
 Imperial College London
 University of Cambridge

Abstract

Background: The available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and L. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species. Methodology and Findings: We modified the existing scheme by replacing one of the seven MLST loci (fadD was changed to caiB), as the former gene did not appear to be present in some pathogenic species. Comparison of the original and modified schemes using data for L. interrogans and L. kirschneri demonstrated that the discriminatory power of the two schemes was not significantly different. The modified scheme was used to further characterize 325 isolates (L. alexanderi [n = 5], L. borgpetersenii [n = 34], L. interrogans [n = 222], L. kirschneri [n = 29], L. noguchii [n = 9], L. santarosai [n = 10], and L. weilii [n = 16]). Phylogenetic analysis using concatenated sequences of the 7 loci demonstrated that each species corresponded to a discrete clade, and that no strains were misclassified at the species level. Comparison between genotype and serovar was possible for 254 isolates. Of the 31 sequence types (STs) represented by at least two isolates, 18 STs included isolates assigned to two or three different serovars. Conversely, 14 serovars were identified that contained between 2 to 10 different STs. New observations were made on the global phylogeography of Leptospira spp., and the utility of MLST in making associations between human disease and specific maintenance hosts was demonstrated. Conclusion: The new MLST scheme, supported by an updated MLST website, allows the characterization and species assignment of isolates of the seven major pathogenic species associated with leptospirosis.

Keyword(s)

Medicine
 Pharmacology, Toxicology and Pharmaceutics

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URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/32708

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Scopus 2011-2015