Publication: Infection of human primary hepatocytes with dengue virus serotype 2
Issued Date
2007-03-01
Resource Type
ISSN
10969071
01466615
01466615
Other identifier(s)
2-s2.0-33846785137
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Medical Virology. Vol.79, No.3 (2007), 300-307
Suggested Citation
Lukkana Suksanpaisan, Arturo Cabrera-Hernandez, Duncan R. Smith Infection of human primary hepatocytes with dengue virus serotype 2. Journal of Medical Virology. Vol.79, No.3 (2007), 300-307. doi:10.1002/jmv.20798 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24568
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Infection of human primary hepatocytes with dengue virus serotype 2
Other Contributor(s)
Abstract
While the impact of the dengue viruses on liver function is prominent as shown by hepatomegaly, liver enzyme abnormality, occasional fulminant hepatic failure and histological changes including hepatocellular necrosis, significant debate exists as to the possible involvement of the predominant cell type in the liver, hepatocytes, in the disease process. To address this issue purified human primary hepatocytes were exposed to dengue virus serotype 2 and the production of de novo viral progeny was established by standard plaque assay, RT-PCR and immunocytochemistry. To investigate the response of the primary hepatocytes to infection, the expression of a panel of 9 cytokine genes (IFN-β, TRAIL, MCP-1, IL-6, IL-1β, IL-8, MIP-1α, MIP-1β, and RANTES) was semi-quantitatively investigated by RT-PCR and up-regulation of TRAIL, MIP-α, IFN-β, MIP-1β, IL-8, and RANTES was observed in response to infection. The induction of IL-8 in response to infection was accompanied by the secretion of IL-8 as verified by ELISA assay. The ability of hepatocytes to be infected with dengue virus serotype 2 in vitro support evidence implicating human hepatocytes as a target cell in cases of dengue virus infection, and provide the first experimental evidence to support the large number of clinical studies that implicate the liver as a critical target organ in severe cases of dengue infection. © 2007 Wiley-Liss, Inc.