Publication: Assay for screening for six antimalarial drugs and one metabolite using dried blood spot sampling, sequential extraction and ion-trap detection
Issued Date
2010-11-01
Resource Type
ISSN
17576199
17576180
17576180
Other identifier(s)
2-s2.0-79952521150
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Bioanalysis. Vol.2, No.11 (2010), 1839-1847
Suggested Citation
Daniel Blessborn, Susanne Romsing, Yngve Bergqvist, Niklas Lindegardh Assay for screening for six antimalarial drugs and one metabolite using dried blood spot sampling, sequential extraction and ion-trap detection. Bioanalysis. Vol.2, No.11 (2010), 1839-1847. doi:10.4155/bio.10.147 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28610
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Title
Assay for screening for six antimalarial drugs and one metabolite using dried blood spot sampling, sequential extraction and ion-trap detection
Abstract
Background: More parasites are becoming resistant to antimalarial drugs, and in many areas a change in first-line drug treatment is necessary. The aim of the developed assay is to help determine drug use in these areas and also to be a complement to interviewing patients, which will increase reliability of surveys. Results: This assay detects quinine, mefloquine, sulfadoxine, pyrimethamine, lumefantrine, chloroquine and its metabolite desethylchloroquine in a 100-μl dried blood spot. Most of the drugs also have long half-lives that make them detectable at least 7 days after administration. The drugs are extracted from the dried blood spot with sequential extraction (due to the big differences in physicochemical properties), solid-phase extraction is used as sample clean-up and separation is performed with gradient-LC with MS ion-trap detection. Conclusion: Detection limits (S/N > 5:1) at 50 ng/ml or better were achieved for all drugs except lumefantrine (200 ng/ml), and thus can be used to determine patient compliance. A major advantage of using the ion-trap MS it that it will be possible to go back into the data and look for other drugs as needed. © 2010 Future Science Ltd.