Publication: Simple method for screening of α-thalassaemia 1 carriers
Issued Date
2009-06-01
Resource Type
ISSN
09255710
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2-s2.0-67749088035
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Hematology. Vol.89, No.5 (2009), 559-567
Suggested Citation
Chatchai Tayapiwatana, Surakit Kuntaruk, Thanusak Tatu, Sawitree Chiampanichayakul, Thongperm Munkongdee, Pranee Winichagoon, Suthat Fuchareon, Watchara Kasinrerk Simple method for screening of α-thalassaemia 1 carriers. International Journal of Hematology. Vol.89, No.5 (2009), 559-567. doi:10.1007/s12185-009-0331-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28063
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Title
Simple method for screening of α-thalassaemia 1 carriers
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Abstract
α-Thalassaemia 1 genetic disorder occurs when there is a deletion of two linked α-globin genes. The interaction between these abnormal genes leads to the most severe type of thalassaemia disease, haemoglobin (Hb) Bart's hydrops fetalis. The identification of α-thalassaemia 1 carriers and genetic counselling are essential for the prevention and control of severe thalassaemia diseases. In this study, we have developed a rapid screening method for identifying α-thalassaemia 1. A sandwich-type immunochromatographic (IC) strip test was developed, using the generated monoclonal anti-Hb Bart's antibody, to trace the Hb Bart's in haemolysates. When assayed by our IC strip test, all α-thalassaemia 1, HbH disease, HbH-Constant Spring (H-CS) disease, HbH-CS and heterozygous HbE (CSEA) Bart's disease, and homozygous α-thalassaemia 2 showed positive results. No false negative results were observed in these blood samples. In α-thalassaemia 2 heterozygotes, 83% of them showed positive reactivity. Among HbE (both homozygotes and heterozygotes), β-thalassaemia (heterozygotes, homozygotes and β-thalassaemia/HbE) and normal subjects, the IC strip test revealed negative reactivity of 100, 85 and 97%, respectively. These results indicate that this novel immunodiagnostic kit, in combination with red blood cell indices, is suitable for screening and ruling out mass populations for the presence of α-thalassaemia 1. © 2009 The Japanese Society of Hematology.