Publication: Qualitative and semiquantitative polymerase chain reaction to predict plasmodium falciparum treatment failure
Issued Date
1994-01-01
Resource Type
ISSN
15376613
00221899
00221899
Other identifier(s)
2-s2.0-0028110225
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Infectious Diseases. Vol.170, No.6 (1994), 1626-1630
Suggested Citation
Kevin C. Kain, Dennis E. Kyle, Chansuda Wongsrichanalai, Arthur E. Brown, H. Kyle Webster, S. Vanijanonta, Sornchai Looareesuwan Qualitative and semiquantitative polymerase chain reaction to predict plasmodium falciparum treatment failure. Journal of Infectious Diseases. Vol.170, No.6 (1994), 1626-1630. doi:10.1093/infdis/170.6.1626 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/9842
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Title
Qualitative and semiquantitative polymerase chain reaction to predict plasmodium falciparum treatment failure
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Abstract
Multidrug-resistant falciparum malaria is increasing in most malaria-endemic areas. Rapid methods for predicting treatment failure would aid management and control of drug-resistant infections. In this study, Plasmodium falciparum DNA clearance was examined by qualitative and semiquantitative polymerase chain reaction (PCR). Thai patients with acute falciparum malaria were prospectively followed by light microscopy and by PCR of P. falciparum DNA eluted from filter paper blood samples. A 206-bp P. falciparum sequence was amplified and detected radiometrically and by high-performance liquid chromatography. Clearance of P.falciparum DNA was significantly delayed in treatment failures compared with that in successfully treated patients (P =.02). Semiquantitative PCR levels did not drop to < 50% of pretreatment levels until day 3 or later in treatment failures compared with day 1 or earlier for successfully treated parasitemia-matched controls (P =.005). These results suggest that qualitative and semiquantitative PCR may be useful as a method for monitoring response to therapy. © 1994 by The University of Chicago.