Publication: Determination and variation of three active diterpenoids in Andrographis paniculata (Burm.f.) Nees
Issued Date
2004-11-01
Resource Type
ISSN
09580344
DOI
Other identifier(s)
2-s2.0-9944239247
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Phytochemical Analysis. Vol.15, No.6 (2004), 365-371
Suggested Citation
Nanthanit Pholphana, Nuchanart Rangkadilok, Sanit Thongnest, Somsak Ruchirawat, Mathuros Ruchirawat, Jutamaad Satayavivad Determination and variation of three active diterpenoids in Andrographis paniculata (Burm.f.) Nees. Phytochemical Analysis. Vol.15, No.6 (2004), 365-371. doi:10.1002/pca.789 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21063
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Determination and variation of three active diterpenoids in Andrographis paniculata (Burm.f.) Nees
Other Contributor(s)
Abstract
Simple and rapid methods have been developed for the extraction and simultaneous determination of the three active diterpenoids, andrographolide (AP 1), 14-deoxy-11,12-didehydroandrographolide (AP 3) and neoandro-grapholide (AP 4) from various samples of Andrographis paniculata (Burm.f.) Nees. Methanol extracts from the dried leaves, stems and crude products were analysed by isocratic HPLC using a methanol and water mobile phase with monitoring at 220 nm. There was a large variation of the three active diterpenoids in different A. paniculata products obtained from Thai markets. The results indicated that the amounts of these active compounds consumed, based on the recommended daily doses, from materials obtained from the different suppliers will be different. In addition, the stability of these three active compounds was also examined in dry herbs stored at room temperature. The results showed that andrographolide was more stable than the others. In contrast, the content of 14-deoxy-11,12-didehydroandrographolide increased and the neoandrographolide content fluctuated during storage time. The combination of different levels of these compounds in the source materials and the changes during storage could have a significant effect on the efficacy of this traditional herbal medicine in clinical treatment. Copyright © 2004 John Wiley & Sons, Ltd.