Publication: Transformation of Palm Oil Mill Effluent to Terpolymer Polyhydroxyalkanoate and Biodiesel Using Rummeliibacillus pycnus Strain TS<inf>8</inf>
Issued Date
2017-01-01
Resource Type
ISSN
1877265X
18772641
18772641
Other identifier(s)
2-s2.0-85001555361
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Waste and Biomass Valorization. Vol.8, No.4 (2017), 1247-1256
Suggested Citation
Pipat Junpadit, Thunwadee Tachapattaworakul Suksaroj, Piyarat Boonsawang Transformation of Palm Oil Mill Effluent to Terpolymer Polyhydroxyalkanoate and Biodiesel Using Rummeliibacillus pycnus Strain TS<inf>8</inf>. Waste and Biomass Valorization. Vol.8, No.4 (2017), 1247-1256. doi:10.1007/s12649-016-9711-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/42539
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Transformation of Palm Oil Mill Effluent to Terpolymer Polyhydroxyalkanoate and Biodiesel Using Rummeliibacillus pycnus Strain TS<inf>8</inf>
Other Contributor(s)
Abstract
© 2016, Springer Science+Business Media Dordrecht. This study aimed to transform wastewater from palm oil mill to the valuable products, especially the terpolymer PHA and biodiesel using Rummeliibacillus pycnus TS8. The cultivation of R. pycnus TS8in POME was conducted in a 1-L aeration reactor. The optimization of bioproducts production was investigated by a statistical method with the central composite rotatable design. The result found that the highest cell dry weight (CDW) and bioproduct accumulation was obtained at the C/N ratio, aeration rate and phosphorus addition of 10, 1.00 vvm and 0.10 g/L, respectively. Moreover, R. pycnus TS8accumulated P (3HB-co-3HV-co-3HHx) with 42.8 mol% 3HB, 34.9 mol% 3HV and 22.38 mol% 3HHx. In addition, R. pycnus TS8also accumulated lipid, especially oleic acid (59.5 % CDW). In addition, the experiment was examined in a 72-L bioreactor to determine the properties of PHA and further used as feedstock for biodiesel production. Afterward, biomass and bioproducts were recovered. The terpolymer PHA and intracellular fatty acid were extracted. The characteristics of terpolymer showed that Tg, Tm, tensile strength, Young’s modulus and elongation at break were −21 °C, 147 °C, 27.67 MPa, 1260 MPa and 11.7 %, respectively. The bioproducts were hydrolyzed to fatty acid methyl ester (FAME) with a heating value, flash point and pour point of 32.9 kJ/g, 132 °C and 7 °C, respectively. The results illustrated that the properties of terpolymer PHA and FAME produced from this study showed a possibility for further application in biopolymer and biodiesel.