Publication: High throughput assay for the determination of lumefantrine in plasma
Issued Date
2005-08-05
Resource Type
ISSN
15700232
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2-s2.0-22244445801
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. Vol.822, No.1-2 (2005), 330-333
Suggested Citation
A. Annerberg, T. Singtoroj, P. Tipmanee, N. J. White, N. P J Day, N. Lindegårdh High throughput assay for the determination of lumefantrine in plasma. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. Vol.822, No.1-2 (2005), 330-333. doi:10.1016/j.jchromb.2005.06.022 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16306
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Title
High throughput assay for the determination of lumefantrine in plasma
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Abstract
A high throughput bioanalytical assay for the determination of lumefantrine in plasma has been developed and validated extensively. The within-day precisions for lumefantrine were 5.2, 3.5 and 2.5% at 200, 2000 and 15000 ng/mL, respectively. The between-day precisions were 4.0, 2.8 and 3.1% at 200, 2000 and 15000 ng/mL, respectively. The lower limits of quantification (LLOQ) and the limits of detection (LOD) were 25 and 10 ng/mL, respectively using 0.250 mL plasma. The average recovery of lumefantrine was 85% and independent upon concentration. The use of 96-well plate format and short chromatographic run has increased the daily sample throughput four times. The assay is particularly suitable for large therapeutic drug monitoring studies using day 7 sampling. © 2005 Elsevier B.V. All rights reserved.