Publication: Differentiation between Mycobacterium tuberculosis and Mycobacterium avium by amplification of the 16S-23S ribosomal DNA spacer
Issued Date
1998-09-01
Resource Type
ISSN
00951137
Other identifier(s)
2-s2.0-0031855110
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Clinical Microbiology. Vol.36, No.9 (1998), 2399-2403
Suggested Citation
Arunnee Sansila, Poonpilas Hongmanee, Charoen Chuchottaworn, Somsak Rienthong, Dhanida Rienthong, Prasit Palittapongarnpim Differentiation between Mycobacterium tuberculosis and Mycobacterium avium by amplification of the 16S-23S ribosomal DNA spacer. Journal of Clinical Microbiology. Vol.36, No.9 (1998), 2399-2403. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18489
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Title
Differentiation between Mycobacterium tuberculosis and Mycobacterium avium by amplification of the 16S-23S ribosomal DNA spacer
Abstract
Differentiation between Mycobacterium tuberculosis and M. avium is helpful for the treatment of disseminated mycobacterial infection in AIDS patients. This can traditionally be done by time-consuming biochemical tests or with Accuprobe. Previously, PCR restriction enzyme analysis (PCR-REA) of the 16S-23S rRNA gene spacer was shown to be able to identify a limited number of strains of Mycobacterium. In this study the method was improved by using more specific primers and was tested with 50 clinical isolates of M. tuberculosis and 65 clinical isolates of M. avium complex. Probes specific to the spacers of M. tuberculosis and M. avium were also tested. Both M. tuberculosis and M. avium could be reliably identified either by PCR-REA or by PCR-hybridization, with the results completely agreeing with those obtained by biochemical tests and with the Accuprobe, respectively. The method may therefore be useful as an alternative in-house method for identification of the bacteria.