Publication:
Development of Hepatocyte-like Cell Derived from Human Induced Pluripotent Stem cell as a Host for Clinically Isolated Hepatitis C Virus

Issued Date

2017-08-14

Resource Type

Article

ISSN

19388969

DOI

10.1002/cpsc.35

Other identifier(s)

2-s2.0-85046264260

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Current protocols in stem cell biology. Vol.42, (2017), 4A.13.1-4A.13.34

Suggested Citation

Khanit Sa-Ngiamsuntorn, Suradej Hongeng, Adisak Wongkajornsilp Development of Hepatocyte-like Cell Derived from Human Induced Pluripotent Stem cell as a Host for Clinically Isolated Hepatitis C Virus. Current protocols in stem cell biology. Vol.42, (2017), 4A.13.1-4A.13.34. doi:10.1002/cpsc.35 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41800

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Thesis

Title

Development of Hepatocyte-like Cell Derived from Human Induced Pluripotent Stem cell as a Host for Clinically Isolated Hepatitis C Virus

Author(s)

Khanit Sa-Ngiamsuntorn
 Suradej Hongeng
 Adisak Wongkajornsilp

Other Contributor(s)

Mahidol University

Abstract

Copyright © 2017 John Wiley & Sons, Inc. This unit describes protocols to develop hepatocyte-like cells (HLCs) starting from mesenchymal stem cells (MSCs) as a natural host for hepatitis C virus (HCV). These include the preparation of MSCs from bone marrow, the reprogramming of MSCs into induced pluripotent stem cells (iPSCs), and the differentiation of iPSCs into HLCs. This unit also incorporates the characterization of the resulting cells at each stage. Another section entails the preparations of HCV. The sources of HCV are either the clinically isolated HCV (HCVser) and the conventional JFH-1 genotype. The last section is the infection protocol coupled with the measurement of viral titer. © 2017 by John Wiley & Sons, Inc.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/41800

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Scopus 2016-2017