Publication: Comparison of different local and imported histamine concentrations used as a skin prick test positive control
Issued Date
2016-12-01
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ISSN
22288694
0125877X
0125877X
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2-s2.0-85008476116
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.34, No.4 (2016), 295-299
Suggested Citation
Nualanong Visitsunthorn, Kittipos Visitsunthorn, Sunisa Aulla, Chaweewan Bunnag, Orathai Jirapongsananuruk Comparison of different local and imported histamine concentrations used as a skin prick test positive control. Asian Pacific Journal of Allergy and Immunology. Vol.34, No.4 (2016), 295-299. doi:10.12932/AP0737 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/40778
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Title
Comparison of different local and imported histamine concentrations used as a skin prick test positive control
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Abstract
© 2016, Allergy and Immunology Society of Thailand. All rights reserved. Background: The skin prick test (SPT) is a valid and approved tool that is used to diagnose atopic diseases. The SPT is accurate, safe, simple and inexpensive. However, the histamine concentration used as a positive control in the SPT varies among centers. Objective: To compare SPT results using different concentrations of locally-prepared and imported histamine solutions. Method: This prospective, randomized, double-blind, self-controlled study was performed in healthy adult volunteers. The SPT was performed using 4 concentrations of histamine solutions (imported, 1 mg/mL; locally-prepared, 1, 5 and 10 mg/mL). Locally-prepared histamine positive controls were prepared from histamine biphosphate monohydrate using sterile technique. Results: Seventy-five adult volunteers (mean age, 36 years) were included in the study. Eight volunteers were male and 9 had a history of atopy. Mean wheal diameter (MWD) for imported histamine was 3.49 mm for a concentration of 1 mg/mL, and that of locally-prepared histamine was 2.94 mm, 5.05 mm and 5.52 mm for concentrations of 1, 5 and 10 mg/mL histamine, respectively. Negative SPT results (MWD <3 mm) were found in 11 subjects (14.7%) who received imported histamine and 26 subjects (34.7%) who received the locally-prepared histamine at concentration of 1 mg/mL. All subjects tested with locally-prepared histamine at concentrations of 5 and 10 mg/mL had a MWD > 3 mm. Conclusions: Locally-prepared histamine base at concentrations of 5 and 10 mg/mL yielded better positive results than both imported and locally-prepared histamine at a concentration of 1 mg/mL.