Publication: Specific B-cell epitope of per a 1: A major allergen of American cockroach (Periplaneta Americana) and anatomical localization
Issued Date
2014-01-01
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20927363
20927355
20927355
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2-s2.0-84903472465
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Mahidol University
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SCOPUS
Bibliographic Citation
Allergy, Asthma and Immunology Research. Vol.6, No.4 (2014), 325-332
Suggested Citation
Nitat Sookrung, Thanyathon Khetsuphan, Urai Chaisri, Nitaya Indrawattana, Onrapak Reamtong, Wanpen Chaicumpa, Anchalee Tungtrongchitr Specific B-cell epitope of per a 1: A major allergen of American cockroach (Periplaneta Americana) and anatomical localization. Allergy, Asthma and Immunology Research. Vol.6, No.4 (2014), 325-332. doi:10.4168/aair.2014.6.4.325 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/34042
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Title
Specific B-cell epitope of per a 1: A major allergen of American cockroach (Periplaneta Americana) and anatomical localization
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Abstract
Purpose: Cockroach (CR) is a common source of indoor allergens, and Per a 1 is a major American CR (Periplaneta americana) allergen; however, several attributes of this protein remain unknown. This study identifies a novel specific B cell epitope and anatomical locations of Per a 1.0105. Methods: Recombinant Per a 1.0105 (rPer a 1.0105) was used as BALB/c mouse immunogen for the production of monoclonal antibodies (MAb). The MAb specific B cell epitope was identified by determining phage mimotopic peptides and pair-wise alignment of the peptides with the rPer a 1.0105 amino acid sequence. Locations of the Per a 1.0105 in P. americana were investigated by immunohistochemical staining. Results: The rPer a 1.0105 (~13 kDa) had 100%, 98% and ≥90% identity to Per a 1.0105, Per a 1.0101, and Cr-PII, respectively. The B-cell epitope of the Per a 1.0105 specific-MAb was located at residues 99 QDLLLQLRDKGV110 contained in all 5 Per a 1.01 isoforms and Per a 1.02. The epitope was analogous to the Bla g 1.02 epitope; however, this B-cell epitope was not an IgE inducer. Per a 1.0105 was found in the midgut and intestinal content of American CR but not in the other organs. The amount of the Per a 1 was ~544 μg per gram of feces. Conclusions: The novel Per a 1 B-cell epitope described in this study is a useful target for allergen quantification in samples; however, the specific MAb can be used as an allergen detection reagent. The MAb based-affinity resin can be made for allergen purification, and the so-purified protein can serve as a standard and diagnostic allergen as well as a therapeutic vaccine component. The finding that the Per a 1 is contained in the midgut and feces is useful to increase yield and purity when preparing this allergen. © Copyright The Korean Academy of Asthma, Allergy and Clinical Immunology.