Publication: The diagnostic accuracy of three rapid diagnostic tests for typhoid fever at Chittagong Medical College Hospital, Chittagong, Bangladesh
Issued Date
2015-10-01
Resource Type
ISSN
13653156
13602276
13602276
Other identifier(s)
2-s2.0-84941040596
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Tropical Medicine and International Health. Vol.20, No.10 (2015), 1376-1384
Suggested Citation
Rapeephan R. Maude, Hanna K. de Jong, Lalith Wijedoru, Masako Fukushima, Aniruddha Ghose, Rasheda Samad, Mohammed Amir Hossain, Mohammed Rezaul Karim, Mohammed Abul Faiz, Christopher M. Parry, Abdullah Abu Sayeed, Mahtab Uddin Hasan, Wirichada Pan-Ngum, Thomas W. van der Vaart, Asok Kumar Dutta, Nasir Uddin Mahmud, Murad Hero, Nafiz Iqbal, Zabeen Chaudhury, Tran Vu Thieu Nga, Pham Thanh Duy, Voong Vinh Phat, Richard J. Maude, Stephen Baker, W. Joost Wiersinga, Tom van der Poll, Nicholas P. Day, Arjen M. Dondorp The diagnostic accuracy of three rapid diagnostic tests for typhoid fever at Chittagong Medical College Hospital, Chittagong, Bangladesh. Tropical Medicine and International Health. Vol.20, No.10 (2015), 1376-1384. doi:10.1111/tmi.12559 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/36068
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Title
The diagnostic accuracy of three rapid diagnostic tests for typhoid fever at Chittagong Medical College Hospital, Chittagong, Bangladesh
Author(s)
Rapeephan R. Maude
Hanna K. de Jong
Lalith Wijedoru
Masako Fukushima
Aniruddha Ghose
Rasheda Samad
Mohammed Amir Hossain
Mohammed Rezaul Karim
Mohammed Abul Faiz
Christopher M. Parry
Abdullah Abu Sayeed
Mahtab Uddin Hasan
Wirichada Pan-Ngum
Thomas W. van der Vaart
Asok Kumar Dutta
Nasir Uddin Mahmud
Murad Hero
Nafiz Iqbal
Zabeen Chaudhury
Tran Vu Thieu Nga
Pham Thanh Duy
Voong Vinh Phat
Richard J. Maude
Stephen Baker
W. Joost Wiersinga
Tom van der Poll
Nicholas P. Day
Arjen M. Dondorp
Hanna K. de Jong
Lalith Wijedoru
Masako Fukushima
Aniruddha Ghose
Rasheda Samad
Mohammed Amir Hossain
Mohammed Rezaul Karim
Mohammed Abul Faiz
Christopher M. Parry
Abdullah Abu Sayeed
Mahtab Uddin Hasan
Wirichada Pan-Ngum
Thomas W. van der Vaart
Asok Kumar Dutta
Nasir Uddin Mahmud
Murad Hero
Nafiz Iqbal
Zabeen Chaudhury
Tran Vu Thieu Nga
Pham Thanh Duy
Voong Vinh Phat
Richard J. Maude
Stephen Baker
W. Joost Wiersinga
Tom van der Poll
Nicholas P. Day
Arjen M. Dondorp
Other Contributor(s)
Mahidol University
Division of Infectious Diseases and Center for Infection and Immunity Amsterdam (CINIMA)
Academic Medical Centre, University of Amsterdam
Liverpool School of Tropical Medicine
Chittagong Medical College Hospital
Centre for Specialized Care and Research
London School of Hygiene & Tropical Medicine
Nagasaki University
Division of Infectious Diseases and Center for Infection and Immunity Amsterdam (CINIMA)
Academic Medical Centre, University of Amsterdam
Liverpool School of Tropical Medicine
Chittagong Medical College Hospital
Centre for Specialized Care and Research
London School of Hygiene & Tropical Medicine
Nagasaki University
Abstract
© 2015 John Wiley & Sons Ltd. Objective: To determine the diagnostic accuracy of three rapid diagnostic tests (RDTs) for typhoid fever in febrile hospitalised patients in Bangladesh. Methods: Febrile adults and children admitted to Chittagong Medical College Hospital, Bangladesh, were investigated with Bact/Alert® blood cultures and real-time PCR to detect Salmonella enterica Typhi and Paratyphi A and assays for Rickettsia, leptospirosis and dengue fever. Acute serum samples were examined with the LifeAssay (LA) Test-it™ Typhoid IgM lateral flow assay detecting IgM antibodies against S. Typhi O antigen, CTKBiotech Onsite Typhoid IgG/IgM Combo Rapid-test cassette lateral flow assay detecting IgG and IgM antibodies against S. Typhi O and H antigens and SD Bioline line assay for IgG and IgM antibodies against S. Typhi proteins. Results: In 300 malaria smear-negative febrile patients [median (IQR) age of 13.5 (5-31) years], 34 (11.3%) had confirmed typhoid fever: 19 positive by blood culture for S. Typhi (three blood PCR positive) and 15 blood culture negative but PCR positive for S. Typhi in blood. The respective sensitivity and specificity of the three RDTs in patients using a composite reference standard of blood culture and/or PCR-confirmed typhoid fever were 59% and 61% for LifeAssay, 59% and 74% for the CTK IgM and/or IgG, and 24% and 96% for the SD Bioline RDT IgM and/or IgG. The LifeAssay RDT had a sensitivity of 63% and a specificity of 91% when modified with a positive cut-off of ≥2+ and analysed using a Bayesian latent class model. Conclusions: These typhoid RDTs demonstrated moderate diagnostic accuracies, and better tests are needed.