Publication: Generation of a WWTR1 mutation induced pluripotent stem cell line, MUSIi012-A-1, using CRISPR/Cas9
Issued Date
2019-12-01
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ISSN
18767753
18735061
18735061
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2-s2.0-85074105106
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Mahidol University
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SCOPUS
Bibliographic Citation
Stem Cell Research. Vol.41, (2019)
Suggested Citation
Chanchao Lorthongpanich, Nittaya Jiamvoraphong, Prapasri Supakun, Nattaya Damkham, Papussorn Terbto, Supaporn Waeteekul, Yaowalak U-pratya, Chuti Laowtammathron, Surapol Issaragrisil Generation of a WWTR1 mutation induced pluripotent stem cell line, MUSIi012-A-1, using CRISPR/Cas9. Stem Cell Research. Vol.41, (2019). doi:10.1016/j.scr.2019.101634 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50007
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Title
Generation of a WWTR1 mutation induced pluripotent stem cell line, MUSIi012-A-1, using CRISPR/Cas9
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Abstract
© 2019 The Author(s) WWTR1 or TAZ (WWTR1/TAZ) is a transcriptional coactivator that acts as a downstream regulatory target in the Hippo signaling pathway, which plays a pivotal role in regulating cell proliferation and anti-apoptosis. It has been shown in other cell types that WWTR1/TAZ plays a redundant role to its homolog YAP1. Using CRISPR/Cas9 gene editing, we established the WWTR1/TAZ-KO cell line, which features homozygous deletion of WWTR1 gene from human iPSCs. The established WWTR1/YAZ-KO cell line maintained the pluripotent phenotype, the ability to differentiate into all three embryonic germ layers, and normal karyotype.