Publication: Nitric oxide synthesis inhibition and anti-inflammatory effect of polypeptide isolated from chicken feather meal in lipopolysaccharide-stimulated RAW 264.7 macrophages
Issued Date
2019-04-01
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13342606
13309862
13309862
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2-s2.0-85069807751
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Mahidol University
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SCOPUS
Bibliographic Citation
Food Technology and Biotechnology. Vol.57, No.2 (2019), 200-211
Suggested Citation
Aurachorn Inkanuwat, Romteera Sukaboon, Onrapak Reamtong, Pravit Asawanonda, Ake Pattaratanakun, Tanatorn Saisavoey, Papassara Sangtanoo, Aphichart Karnchanatat Nitric oxide synthesis inhibition and anti-inflammatory effect of polypeptide isolated from chicken feather meal in lipopolysaccharide-stimulated RAW 264.7 macrophages. Food Technology and Biotechnology. Vol.57, No.2 (2019), 200-211. doi:10.17113/ftb.57.02.19.5964 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/49790
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Title
Nitric oxide synthesis inhibition and anti-inflammatory effect of polypeptide isolated from chicken feather meal in lipopolysaccharide-stimulated RAW 264.7 macrophages
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Abstract
© 2019 University of Zagreb. Nitric oxide (NO) plays a key role in the pathogenesis of inflammation and has been implicated in endotoxin-induced tissue injury. Chicken feather meal is a rich source of amino acids that may serve as a peptide hydrolysate to inhibit NO activity. Anti-inflammatory hydrolysates of chicken feather meal were prepared and fractionated into five samples based on molecular mass. The smallest fraction (<0.65 kDa) exhibited the highest NO inhibitory activity without cytotoxicity towards macrophage RAW 264.7 cells. Further subfractions were sufficient to obtain amino acid sequences by Q-TOF LC-MS/MS ESI analysis. Of these, the SNPSVAGVR (885.97 Da) peptide and its corresponding pure synthetic peptide have inhibitory activity against NO production by RAW 264.7 cells (IC50=(55.2±0.2) mM) without cytotoxicity. Reverse transcriptase polymerase chain reaction (RT-PCR) and quantitative real-time RT-PCR results revealed that the peptide of the obtained fraction reduced transcript expression levels of the pro-inflammatory cytokines iNOS, TNF-a, COX-2 and IL-6 in lipopolysaccharide-stimulated RAW 264.7 cells. These results suggest that the peptides derived from the chicken feather meal protein could potentially be used as a promising ingredient in functional foods or nutraceuticals against inflammatory diseases.
