Publication: A noradrenergic sensitive endogenous clock is present in the rat pineal gland
2
item.page.dc.date.issued
2011-07-01
item.page.dc.type
item.page.dc.identifier.issn
14230194
00283835
00283835
item.page.dc.identifier.doi
item.page.dc.identifier.other
2-s2.0-79960932529
item.page.dc.rights
Mahidol University
item.page.dc.rights.holder
SCOPUS
item.page.dc.identifier.citation
Neuroendocrinology. Vol.94, No.1 (2011), 75-83
punsarn.item.page.citation
Prapimpun Wongchitrat, Marie Paule Felder-Schmittbuhl, Piyarat Govitrapong, Pansiri Phansuwan-Pujito, Valérie Simonneaux A noradrenergic sensitive endogenous clock is present in the rat pineal gland. Neuroendocrinology. Vol.94, No.1 (2011), 75-83. doi:10.1159/000327430 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/11524
Research Projects
Organizational Units
Authors
Journal Issue
relationships.isThesisOf
item.page.dc.title
A noradrenergic sensitive endogenous clock is present in the rat pineal gland
item.page.dc.contributor.author
item.page.dc.contributor.other
item.page.dc.description.abstract
The aim of this study was to examine the occurrence of endogenous oscillations of Per1, Per2, Bmal1 and Rev-erbα genes in rat pineal explants and to investigate their regulation by adrenergic ligands. Our results show a significant and sustained rhythm of Per2,Bmal1 and Rev-erbα gene expression for up to 48 h in cultured pineal gland with a pattern similar to that observed in vivo. By contrast, the rhythms of Per1 and Aa-nat, the rate-limiting enzyme for melatonin synthesis, were strongly attenuated after 24 h in culture. Addition of the exogenous adrenergic agonist isoproterenol on cultured pineal glands induced a short-term increase in mRNA levels of Per1 and Aa-nat, but not those of Per2,Bmal1 and Rev-erbα. This study demonstrates that the rat pineal gland hosts a circadian oscillator as evidenced by the sustained, noradrenergic-independent, endogenous oscillations of Per2, Bmal1 and Rev-erbα mRNA levels in cultured tissues. Only expression of Per1 was stimulated by adrenergic ligands suggesting that, in vivo, the adrenergic input could synchronize the pineal clock by acting selectively on Per1. Copyright © 2011 S. Karger AG, Basel.