Publication: Improved culture-based detection and quantification of Burkholderia pseudomallei from soil
2
Issued Date
2011-06-01
Resource Type
ISSN
18783503
00359203
00359203
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2-s2.0-79956074941
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Mahidol University
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SCOPUS
Bibliographic Citation
Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.105, No.6 (2011), 346-351
Suggested Citation
Trinh Thanh Trung, Adrian Hetzer, Eylin Topfstedt, Andre Göhler, Direk Limmathurotsakul, Vanaporn Wuthiekanun, Sharon J. Peacock, Ivo Steinmetz Improved culture-based detection and quantification of Burkholderia pseudomallei from soil. Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.105, No.6 (2011), 346-351. doi:10.1016/j.trstmh.2011.03.004 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/12039
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Title
Improved culture-based detection and quantification of Burkholderia pseudomallei from soil
Other Contributor(s)
Abstract
Environmental surveillance of the Gram-negative soil bacterium Burkholderia pseudomallei, the aetiological agent of melioidosis, is important in order to define human populations and livestock at risk of acquiring the infection. This study aimed to develop a more sensitive method for the detection of B. pseudomallei from soil samples in endemic areas compared with the currently used culture method based on soil dispersion in water. We report the development of a new protocol that involves soil dispersion in a polyethylene glycol (PEG) and sodium deoxycholate (DOC) solution to increase the yield of viable B. pseudomallei from soil samples. Comparative testing of soil samples from Northeast Thailand covering a w ide range of B. pseudomallei concentrations demonstrated a significantly higher recovery (P < 0.0001) of B. pseudomallei colony-forming units by the new method compared with the conventional method. The data indicate that using the detergents PEG and DOC not only results in a higher recovery of viable B. pseudomallei but also results in a shift in the bacterial species recovered from soil samples. Future studies on the geographical distribution and population structure of B. pseudomallei in soil are likely to benefit from the new protocol described here. © 2011 Royal Society of Tropical Medicine and Hygiene.