Publication: Evidence to support a conspecific nature of allopatric cytological races of Anopheles nitidus (Diptera: Culicidae) in Thailand
Issued Date
2014-01-01
Resource Type
ISSN
15362442
Other identifier(s)
2-s2.0-84994000680
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Insect Science. Vol.14, No.1 (2014)
Suggested Citation
Siripan Songsawatkiat, Visut Baimai, Sorawat Thongsahuan, Yasushi Otsuka, Kritsana Taai, Chayanit Hempolchom, Wichai Srisuka, Petchaboon Poolphol, Wej Choochote, Atiporn Saeung, Igor Sharkhov Evidence to support a conspecific nature of allopatric cytological races of Anopheles nitidus (Diptera: Culicidae) in Thailand. Journal of Insect Science. Vol.14, No.1 (2014). doi:10.1093/jisesa/ieu149 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33141
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Evidence to support a conspecific nature of allopatric cytological races of Anopheles nitidus (Diptera: Culicidae) in Thailand
Abstract
© The Author 2014. Metaphase karyotype investigation on two allopatric strains of Anopheles nitidus Harrison, Scanlon, and Reid (Diptera: Culicidae) was conducted in Thailand during 2011-2012. Five karyotypic forms, i.e., Form A (X1, Y1), Form B (X1, Y2), Form C (X2, Y3), Form D (X1, X3, Y4), and Form E (X1, X2, X3, Y5) were obtained from a total of 21 isofemale lines. Forms A, B, and C were confined to Phang Nga Province, southern Thailand, whereas Forms D and E were restricted to Ubon Ratchathani Province, northeastern Thailand. Cross-mating experiments among the five isofemale lines, which were representative of five karyotypic forms of An. nitidus, revealed genetic compatibility by providing viable progenies and synaptic salivary gland polytene chromosomes through F2generations. The results suggest that the forms are conspecific, and An. nitidus comprises five cytological races. The very low intraspecific sequence variations (average genetic distances = 0.002-0.008) of the nucleotide sequences in ribosomal DNA (internal transcribed spacer 2) and mitochondrial DNA (cytochrome c oxidase subunits I and II) among the five karyotypic forms were very good supportive evidence.