Publication: Pinus maritima Extract Induces Apoptosis in Human Malignant Melanoma Cells via ROS/Caspase-3 Signaling
Issued Date
2020-01-01
Resource Type
ISSN
15559475
1934578X
1934578X
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2-s2.0-85087946857
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Mahidol University
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SCOPUS
Bibliographic Citation
Natural Product Communications. Vol.15, No.5 (2020)
Suggested Citation
Sunisa Thaichinda, Salunya Tancharoen, Takuro Kanekura, Yuko Higashi, Pornpen Dararat, Kiyoshi Kikuchi, Thamthiwat Nararatwanchai Pinus maritima Extract Induces Apoptosis in Human Malignant Melanoma Cells via ROS/Caspase-3 Signaling. Natural Product Communications. Vol.15, No.5 (2020). doi:10.1177/1934578X20926889 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/57646
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Title
Pinus maritima Extract Induces Apoptosis in Human Malignant Melanoma Cells via ROS/Caspase-3 Signaling
Abstract
© The Author(s) 2020. Melanoma is the most aggressive type of skin cancer due to its rapid metastasis with a high recurrence rate following conventional therapy. Pine bark extract (PBE) from Pinus maritima contains numerous phenolic compounds and functions as a potent antioxidant. The present study aimed to analyze the potential anticancer properties of PBE on human malignant melanoma A375 cells. The chemical composition of PBE was determined by high-performance liquid chromatography/photodiode array detector. The effects of PBE on cell death, migration, and invasion were determined using xCELLigence Technology real-time cell analysis. Annexin/propidium iodide flow cytometry and Hoechst 33342 staining were conducted to detect cell apoptosis. PBE induced apoptosis and inhibited cell migration and invasion. Cleaved caspase-3 expression and activity were significantly increased (P < 0.01) in cells treated with PBE compared with control cells. PBE ameliorated hydrogen peroxide (H2O2)-induced reactive oxygen species (ROS) formation. Treatment of the cells with PBE in the presence of H2O2 led to significant (P < 0.001) reduction of matrix metallopeptidase-9, which is a mediator responsible for advanced melanoma. PBE induces A375 programmed cell death and suppresses cellular invasion by attenuating the ROS-dependent pathway associated with MMP-9 reduction.