Publication: Localization of α-glucosidases I, II, and III in organs of European honeybees, Apis mellifera L., and the origin of α-glucosidase in honey
Issued Date
2004-11-01
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ISSN
09168451
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2-s2.0-19944387600
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Mahidol University
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SCOPUS
Bibliographic Citation
Bioscience, Biotechnology and Biochemistry. Vol.68, No.11 (2004), 2346-2352
Suggested Citation
Masaki Kubota, Masahisa Tsuji, Mamoru Nishimoto, Jintanart Wongchawalit, Masayuki Okuyama, Haruhide Mori, Hirokazu Matsui, Rudee Surarit, Jisnuson Svasti, Atsuo Kimura, Seiya Chiba Localization of α-glucosidases I, II, and III in organs of European honeybees, Apis mellifera L., and the origin of α-glucosidase in honey. Bioscience, Biotechnology and Biochemistry. Vol.68, No.11 (2004), 2346-2352. doi:10.1271/bbb.68.2346 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21127
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Title
Localization of α-glucosidases I, II, and III in organs of European honeybees, Apis mellifera L., and the origin of α-glucosidase in honey
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Abstract
Three kinds of α-glucosidases, I, II, and III, were purified from European honeybees, Apis mellifera L. In addition, an α-glucosidase was also purified from honey. Some properties, including the substrate specificity of honey α-glucosidase, were almost the same as those of α-glucosidase III. Specific antisera against the α-glucosidases were prepared to examine the localization of α-glucosidases in the organs of honeybees. It was immunologically confirmed for the first time that α-glucosidase I was present in ventriculus, and α-glucosidase II, in ventriculus and haemolymph. α-Glucosidase III, which became apparent to be honey α-glucosidase, was present in the hypopharyngeal gland, from which the enzyme may be secreted into nectar gathered by honeybees. Honey may be finally made up through the process whereby sucrose in nectar, in which glucose and fructose also are naturally contained, is hydrolyzed by secreted α-glucosidase III.