Publication: Shigella MreB promotes polar IcsA positioning for actin tail formation
Issued Date
2019-05-01
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ISSN
14779137
00219533
00219533
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2-s2.0-85065535708
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Cell Science. Vol.132, No.9 (2019)
Suggested Citation
Sina Krokowski, Sharanjeet Atwal, Damián Lobato-Márquez, Arnaud Chastanet, Rut Carballido-López, Jeanne Salje, Serge Mostowy Shigella MreB promotes polar IcsA positioning for actin tail formation. Journal of Cell Science. Vol.132, No.9 (2019). doi:10.1242/jcs.226217 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50194
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Title
Shigella MreB promotes polar IcsA positioning for actin tail formation
Abstract
© 2019. Published by The Company of Biologists Ltd. Pathogenic Shigella bacteria are a paradigm to address key issues of cell and infection biology. Polar localisation of the Shigella autotransporter protein IcsA is essential for actin tail formation, which is necessary for the bacterium to travel from cell-to-cell; yet how proteins are targeted to the bacterial cell pole is poorly understood. The bacterial actin homologue MreB has been extensively studied in broth culture using model organisms including Escherichia coli, Bacillus subtilis and Caulobacter crescentus, but has never been visualised in rod-shaped pathogenic bacteria during infection of host cells. Here, using single-cell analysis of intracellular Shigella, we discover that MreB accumulates at the cell pole of bacteria forming actin tails, where it colocalises with IcsA. Pharmacological inhibition of host cell actin polymerisation and genetic deletion of IcsA is used to show, respectively, that localisation of MreB to the cell poles precedes actin tail formation and polar localisation of IcsA. Finally, by exploiting the MreB inhibitors A22 and MP265, we demonstrate that MreB polymerisation can support actin tail formation. We conclude that Shigella MreB promotes polar IcsA positioning for actin tail formation, and suggest that understanding the bacterial cytoskeleton during host–pathogen interactions can inspire development of new therapeutic regimes for infection control.