Publication: Worldwide dissemination of acquired carbapenem-hydrolysing class D β-lactamases in Acinetobacter spp. other than Acinetobacter baumannii
Issued Date
2014-01-01
Resource Type
ISSN
18727913
09248579
09248579
Other identifier(s)
2-s2.0-84897985943
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Antimicrobial Agents. Vol.43, No.4 (2014), 375-377
Suggested Citation
Esther Zander, Ana Fernández-González, Xenia Schleicher, Cathrin Dammhayn, Witchuda Kamolvit, Harald Seifert, Paul G. Higgins Worldwide dissemination of acquired carbapenem-hydrolysing class D β-lactamases in Acinetobacter spp. other than Acinetobacter baumannii. International Journal of Antimicrobial Agents. Vol.43, No.4 (2014), 375-377. doi:10.1016/j.ijantimicag.2014.01.012 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/34799
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Title
Worldwide dissemination of acquired carbapenem-hydrolysing class D β-lactamases in Acinetobacter spp. other than Acinetobacter baumannii
Abstract
The aim of this study was to identify acquired OXA-type carbapenemases in Acinetobacter spp. other than Acinetobacter baumannii. From a total of 453 carbapenem-susceptible and -resistant Acinetobacter isolates collected worldwide, 23 were positive for blaOXAgenes by multiplex PCR. These isolates were identified as Acinetobacter pittii (n = 18), Acinetobacter nosocomialis (n = 2), Acinetobacter junii (n = 1) and Acinetobacter genomic species 14TU/13BJ (n = 2). The blaOXAgenes and associated insertion sequence (IS) elements were sequenced by primer walking. In 11 of these isolates, sequencing of the PCR products revealed that they were false-positive for blaOXA. The remaining 12 isolates, originating from Europe, Asia, South America, North America and South Africa, harboured OXA-23 (n = 4), OXA-58 (n = 5), OXA-40-like (n = 1) and OXA-143-like (n = 1); one A. pittii isolate harboured both OXA-23 and OXA-58. IS elements were associated with blaOXAin 10 isolates. OXA multiplex PCR showed a high degree of false-positive results (47.8%), indicating that detection of blaOXAin non-baumannii Acinetobacter spp. should be confirmed using additional methods. © 2014 Elsevier B.V. and the International Society of Chemotherapy.