Publication: Characterization of microsatellite markers in cassava based on microsatellite-AFLP technique
Issued Date
2012-01-01
Resource Type
ISSN
16765680
Other identifier(s)
2-s2.0-84930486032
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Genetics and Molecular Research. Vol.11, No.2 (2012), 1319-1326
Suggested Citation
S. Whankaew, S. Sraphet, R. Thaikert, D. R. Smith, K. Triwitayakorn Characterization of microsatellite markers in cassava based on microsatellite-AFLP technique. Genetics and Molecular Research. Vol.11, No.2 (2012), 1319-1326. doi:10.4238/2012.May.15.2 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13830
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Characterization of microsatellite markers in cassava based on microsatellite-AFLP technique
Other Contributor(s)
Abstract
We developed molecular markers for cassava based on the microsatellite-amplified fragment length polymorphism (M-AFLP) technique. Twenty primer pairs were developed and used for the analysis of 48 samples of Manihot species, consisting of M. esculenta (33), M. esculenta ssp flabellifolia (3), M. chlorosticta (3), M. carthaginensis (3), M. filamentosa (3), and M. tristis (3). Nine microsatellite loci that were polymorphic among these Manihot species were identified, giving 32 polymorphic alleles and from two to seven alleles per locus. Unbiased and direct count heterozygosity varied from 0.0233 to 0.7924 and 0.0000 to 0.7083, respectively. There was significant deviation (P < 0.05) from Hardy-Weinberg equilibrium at five loci. Genotypic data from the Manihot species were subjected to genetic diversity analysis. We found that M. chlorosticta and M. esculenta ssp flabellifolia were the closest populations, while M. filamentosa and M. esculenta ssp flabellifolia were the most divergent. Considering within M. esculenta, the samples from Nigeria and Fiji were the most closely related, while those from Venezuela and of unknown origin were the most divergent. We conclude that the M-AFLP technique is an effective method for generating microsatellite markers that are useful for genetic diversity analysis in Manihot species. © FUNPEC-RP.