Publication: Agrobacterium tumefaciens estC, encoding an enzyme containing esterase activity, Is Regulated by EstR, a regulator in the MarR family
Issued Date
2016-12-01
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ISSN
19326203
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2-s2.0-85007557057
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Mahidol University
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SCOPUS
Bibliographic Citation
PLoS ONE. Vol.11, No.12 (2016)
Suggested Citation
Surawach Rittiroongrad, Nisanart Charoenlap, Suparat Giengkam, Paiboon Vattanaviboon, Skorn Mongkolsuk Agrobacterium tumefaciens estC, encoding an enzyme containing esterase activity, Is Regulated by EstR, a regulator in the MarR family. PLoS ONE. Vol.11, No.12 (2016). doi:10.1371/journal.pone.0168791 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/40681
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Title
Agrobacterium tumefaciens estC, encoding an enzyme containing esterase activity, Is Regulated by EstR, a regulator in the MarR family
Abstract
© 2016 Rittiroongrad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Analysis of the A. tumefaciens genome revealed estC, which encodes an esterase located next to its transcriptional regulator estR, a regulator of esterase in the MarR family. Inactivation of estC results in a small increase in the resistance to organic hydroperoxides, whereas a high level of expression of estC from an expression vector leads to a reduction in the resistance to organic hydroperoxides and menadione. The estC gene is transcribed divergently from its regulator, estR. Expression analysis showed that only high concentrations of cumene hydroperoxide (CHP, 1 mM) induced expression of both genes in an EstR-dependent manner. The EstR protein acts as a CHP sensor and a transcriptional repressor of both genes. EstR specifically binds to the operator sites OI and OII overlapping the promoter elements of estC and estR. This binding is responsible for transcription repression of both genes. Exposure to organic hydroperoxide results in oxidation of the sensing cysteine (Cys16) residue of EstR, leading to a release of the oxidized repressor from the operator sites, thereby allowing transcription and high levels of expression of both genes. The estC is the first organic hydroperoxide-inducible esterase-encoding gene in alphaproteobacteria.