Publication: Biophysical approach to investigate temperature effects on protein dynamics
Issued Date
2015-08-01
Resource Type
ISSN
12860050
12860042
12860042
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2-s2.0-84939435580
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Mahidol University
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SCOPUS
Bibliographic Citation
EPJ Applied Physics. Vol.71, No.3 (2015)
Suggested Citation
Yaowapa Saengpayab, Pisan Kanthang, Stefan Schreier, Charin Modchang, Narin Nuttavut, Darapond Triampo, Wannapong Triampo Biophysical approach to investigate temperature effects on protein dynamics. EPJ Applied Physics. Vol.71, No.3 (2015). doi:10.1051/epjap/2015150180 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/36187
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Title
Biophysical approach to investigate temperature effects on protein dynamics
Abstract
© EDP Sciences, 2015. The main purpose of this work is to gain more understanding of the temperature dependence of Min protein dynamics employing spot tracking technique (STT) and biophysical characterization. We observed and confirmed the variation of protein cluster dynamics at evaluated temperatures. We found that the time MinD was retained at the cell poles played an important role in this variation. From 25 °C to 37 °C, the MinD protein oscillation period decreased 2.3 times and the anomalous dynamic exponent increased 2.4 times. The time-varied anomalous diffusion coefficient was found to be temperature dependent, which was qualitatively consistent with the prediction by Tsallis statistical mechanics. Furthermore, the average apparent effective potential depth of membrane-bound MinD protein decreased from 10.01 to 3.54 k<inf>B</inf>T. These results showed that the diffusive mode and the MinD protein cluster distribution at the cell poles were altered with temperature and this then affected the whole Min protein dynamics.