Publication: Exosome release of β-catenin: A novel mechanism that antagonizes Wnt signaling
Issued Date
2010-09-20
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ISSN
00219525
00219525
00219525
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2-s2.0-77957195392
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Cell Biology. Vol.190, No.6 (2010), 1079-1091
Suggested Citation
Arthit Chairoungdua, Danielle L. Smith, Pierre Pochard, Michael Hull, Michael J. Caplan Exosome release of β-catenin: A novel mechanism that antagonizes Wnt signaling. Journal of Cell Biology. Vol.190, No.6 (2010), 1079-1091. doi:10.1083/jcb.201002049 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/28637
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Title
Exosome release of β-catenin: A novel mechanism that antagonizes Wnt signaling
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Abstract
CD82 and CD9 are tetraspanin membrane proteins that can function as suppressors of tumor metastasis. Expression of CD9 and CD82 in transfected cells strongly suppresses β-catenin - mediated Wnt signaling activity and induces a significant decrease in β-catenin protein levels. Inhibition of Wnt/β-catenin signaling is independent of glycogen synthase kinase-3β and of the proteasome- and lysosome-mediated protein degradation pathways. CD82 and CD9 expression induces β-catenin export via exosomes, which is blocked by a sphingomyelinase inhibitor, GW4869. CD82 fails to induce exosome release of β-catenin in cells that express low levels of E-cadherin. Exosome release from dendritic cells generated from CD9 knockout mice is reduced compared with that from wild-type dendritic cells. These results suggest that CD82 and CD9 down-regulate the Wnt signaling pathway through the exosomal discharge of β-catenin. Thus, exosomal packaging and release of cytosolic proteins can modulate the activity of cellular signaling pathways. © 2010 Chairoungdua et al.