Publication: Purification and characterization of lipase from a raw-milk yeast (Trichosporon asteroides)
Issued Date
1997-10-23
Resource Type
ISSN
08854513
Other identifier(s)
2-s2.0-0030802019
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biotechnology and Applied Biochemistry. Vol.26, No.2 (1997), 111-116
Suggested Citation
Saovanee Dharmsthiti, Palanee Ammaranond Purification and characterization of lipase from a raw-milk yeast (Trichosporon asteroides). Biotechnology and Applied Biochemistry. Vol.26, No.2 (1997), 111-116. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17878
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Purification and characterization of lipase from a raw-milk yeast (Trichosporon asteroides)
Author(s)
Other Contributor(s)
Abstract
A lipase-producing yeast strain was isolated from raw milk. It was identified as Trichosporon asteroides strain LP005. The lipase from this yeast was purified 8-fold to homogeneity for further characterization. The purification process for this lipase included (NH4)2S04 precipitation at 70% saturation and gel filtration on Sephadex G-200. The molecular mass of the lipase was 37 kDa as determined by SDS/PAGE. The optimum pH and temperature for activity were pH 5.0 and 60°C. The lipase was stable over a wide pH range (3.0-10.0) and at temperatures lower than 70°C. The chelating agent EDTA did not affect activity of the enzyme, and this suggested that it was not a metalloenzyme. Treatment of tuna oil with T. asteroides LP005 lipase gave approx. 35 and 47% increases in the concentration of eicosapentaenoic acid and docosahexaenoic acid respectively. Thus, this lipase could potentially be used for the concentration step in the production process of such polyunsatuarated fatty acids.