Publication: Synthesis and theoretical study of molecularly imprinted nanospheres for recognition of tocopherols
Issued Date
2009
Resource Type
Language
eng
ISSN
1420-3049
Rights
Mahidol University
Bibliographic Citation
Molecules. Vol.14, No.8 (2009), 2985-3002
Suggested Citation
Theeraphon Piacham, Chanin Nantasenamat, Thummaruk Suksrichavalit, Charoenchai Puttipanyalears, Tippawan Pissawong, Supanee Maneewas, Chartchalerm Isarankura-Na-Ayudhya, Virapong Prachayasittikul Synthesis and theoretical study of molecularly imprinted nanospheres for recognition of tocopherols. Molecules. Vol.14, No.8 (2009), 2985-3002. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/2114
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Title
Synthesis and theoretical study of molecularly imprinted nanospheres for recognition of tocopherols
Abstract
Molecular imprinting is a technology that facilitates the production of artificial
receptors toward compounds of interest. The molecularly imprinted polymers act as
artificial antibodies, artificial receptors, or artificial enzymes with the added benefit over
their biological counterparts of being highly durable. In this study, we prepared molecularly
imprinted polymers for the purpose of binding specifically to tocopherol (vitamin E) and its
derivative, tocopherol acetate. Binding of the imprinted polymers to the template was found
to be two times greater than that of the control, non-imprinted polymers, when using only 10
mg of polymers. Optimization of the rebinding solvent indicated that ethanol-water at a
molar ratio of 6:4 (v/v) was the best solvent system as it enhanced the rebinding
performance of the imprinted polymers toward both tocopherol and tocopherol acetate with
a binding capacity of approximately 2 mg/g of polymer. Furthermore, imprinted
nanospheres against tocopherol was successfully prepared by precipitation polymerization
with ethanol-water at a molar ratio of 8:2 (v/v) as the optimal rebinding solvent. Computer
simulation was also performed to provide mechanistic insights on the binding mode of
template-monomer complexes. Such polymers show high potential for industrial and
medical applications, particularly for selective separation of tocopherol and derivatives.