Publication: Evaluation of rapid igg4 test for diagnosis of gnathostomiasis
Issued Date
2021-06-01
Resource Type
ISSN
17380006
00234001
00234001
Other identifier(s)
2-s2.0-85110030474
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Korean Journal of Parasitology. Vol.59, No.3 (2021), 257-263
Suggested Citation
Yue Wang, An Ma, Xiao Long Liu, Praphathip Eamsobhana, Xiao Xian Gan Evaluation of rapid igg4 test for diagnosis of gnathostomiasis. Korean Journal of Parasitology. Vol.59, No.3 (2021), 257-263. doi:10.3347/kjp.2021.59.3.257 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/77271
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Evaluation of rapid igg4 test for diagnosis of gnathostomiasis
Other Contributor(s)
Abstract
Human gnathostomiasis is a parasitic disease caused by Gnathostoma nematode infection. A rapid, reliable, and practical immunoassay, named dot immuno-gold filtration assay (DIGFA), was developed to supporting clinical diagnosis of gnathostomiasis. The practical tool detected anti-Gnathostoma-specific IgG4 in human serum using crude ex-tract of third-stage larvae as antigen. The result of the test was shown by anti-human IgG4 monoclonal antibody conju-gated colloidal gold. The sensitivity and specificity of the test were both 100% for detection in human sera from patients with gnathostomiasis (13/13) and from healthy negative controls (50/50), respectively. Cross-reactivity with heterogonous serum samples from patients with other helminthiases ranged from 0 (trichinosis, paragonimiasis, clonorchiasis, schisto-somiasis, and cysticercosis) to 25.0% (sparganosis), with an average of 6.3% (7/112). Moreover, specific IgG4 antibodies diminished at 6 months after treatment. This study showed that DIGFA for the detection of specific IgG4 in human sera could be a promising tool for the diagnosis of gnathostomiasis and useful for evaluating therapeutic effects.