Publication:
Proteomic analysis of serum and urine of HIV-Monoinfected and HIV/HCV-Coinfected patients undergoing long term treatment with Nevirapine

dc.contributor.authorJeerang Wongtrakulen_US
dc.contributor.authorThananya Thongtanen_US
dc.contributor.authorSittiruk Roytrakulen_US
dc.contributor.authorBenjawan Kumrapichen_US
dc.contributor.authorKanokwan Janphenen_US
dc.contributor.authorJutarat Praparattanapanen_US
dc.contributor.authorKhuanchai Supparatpinyoen_US
dc.contributor.authorDuncan R. Smithen_US
dc.contributor.otherMahidol University. Institute of Molecular Biosciencesen_US
dc.date.accessioned2016-04-12T03:07:37Z
dc.date.accessioned2017-04-25T04:07:32Z
dc.date.available2016-04-12T03:07:37Z
dc.date.available2017-04-25T04:07:32Z
dc.date.created2016-04-12
dc.date.issued2014
dc.description.abstractNevirapine (NVP) is an effective nonnucleoside reverse transcriptase inhibitor (NNRTI) of particular interest as it is often used in resource limited countries. However, one of the main concerns with the use of NVP is hepatotoxicity and elevation of liver enzymes as a consequence of highly active antiretroviral therapy (HAART) containing NVP is more often reported in HIV patients coinfected with hepatitis C virus than in HIV-monoinfected patients. To discover possible markers of NVP induced hepatotoxicity, serum and urine samples from twenty-five HIV or HIV/HCV patients, all of whom had received NVP continuously for at least four months, and healthy controls were subjected to in-solution or in-gel proteomic analysis. A total of 83 differentially regulated proteins consisted of 34 proteins identified in serum by in-solution analysis, 2 proteins identified from serum in a 2D gel electrophoresis analysis, and 47 proteins identified in urine in an in-solution analysis. Three proteins, namely, haptoglobin, Rho-related BTB domain containing protein 3, and death-associated protein kinase 3, were selected for further validation by Western blot analysis and results showed that haptoglobin has potential for further development as an additional marker of NVP induced hepatotoxicity.en_US
dc.identifier.citationWongtrakul J, Thongtan T, Roytrakul S, Kumrapich B, Janphen K, Praparattanapan J, et al. Proteomic analysis of serum and urine of HIV-Monoinfected and HIV/HCV-Coinfected patients undergoing long term treatment with Nevirapine. Disease Markers. 2014. doi.10.1155/2014/315824. Article ID 315824.en_US
dc.identifier.doi10.1155/2014/315824
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/1915
dc.language.isoengen_US
dc.rightsMahidol Universityen_US
dc.subjectProteomic Analysisen_US
dc.subjectSerum and Urineen_US
dc.subjectHIV-Monoinfecteden_US
dc.subjectHIV/HCVen_US
dc.subjectCoinfected Patientsen_US
dc.subjectNevirapineen_US
dc.subjectOpen Access articleen_US
dc.titleProteomic analysis of serum and urine of HIV-Monoinfected and HIV/HCV-Coinfected patients undergoing long term treatment with Nevirapineen_US
dc.typeResearch Articleen_US
dcterms.dateAccepted2014-11-25
dspace.entity.typePublication
mods.location.urlhttp://downloads.hindawi.com/journals/dm/2014/315824.pdf

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